Influence of 6-Benzylaminopurine (BAP) and Indole-3-butyric acid (IBA) on shoot and root inductions of Curcuma comosa Roxb. through in vitro culture
Keywords:
Micropropagation, Plant growth regulators, BAP, IBAAbstract
Wan chak mod look (Curcuma comosa Roxb.) belongs to the Zingiberaceae contained bioactive compounds to cure the disorder symptoms of human. However, the wan chak mod look used long period for cultivation, harvesting and propagation. Therefore, the objective of this research was to evaluate the effect of 6-Benzylaminopurine (BAP) and Indole-3-butyric acid (IBA) on micropropagation of wan chak mod look. This research was divided into two experiments 1) effect of different 6-benzylaminopurine concentrations (0, 1, 2 and 3 mg L-1) on shoot induction and 2) effect of different indole-3-butyric acid concentrations (0, 0.5 and 1 mg L-1) on root induction. The experimental design was as Completely Randomized Design (CRD) with 5 replications per treatment. From the result showed that the greatest of shoot number of wan chak mod look when cultured on Murashige and Skoog (MS) medium supplemented with 3 mg L-1 BAP (2.00±0.00 shoots plant-1), but it was not statistically significant difference with MS medium supplemented 1 and 2 mg L-1 BAP. Addition, the leaf number of wan chak mod look was highest when cultured on MS medium supplemented with 3 mg L-1 BAP at 3.00 leaves plant-1, but there was no statistically difference with MS medium supplemented 2 mg L-1 BAP. While, the greatest of shoot height was found at MS medium without BAP (7.50±0.29 cm), but it was no statistically difference with MS medium supplemented with 1 mg L-1 BAP. In addition, the wan chak mod look cultured on MS medium without BAP showed the greatest of leaf length (7.00±0.58 cm), but it was no statistically difference with MS medium supplemented with 1 and 2 mg L-1 BAP. The wan chak mod look cultured on MS medium supplemented with different IBA concentrations (0, 0.5 and 1 mg L-1) did not affect the shoot number, height, root number and root length. The range of shoot number, shoot height, root number and root length were expressed at 1.21±0.15-1.67±0.17 shoots plant-1, 4.17±0.17-4.67±0.44 cm, 2.33±1.45-6.00±0.00 roots plant-1 and 2.33±1.20-6.33±1.09 cm, respectively.
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