The antioxidant ability of juice extracts from Dregea, Gymnema, and Tiliacora leaves by DPPH assay and the production of dry extracts
Keywords:
Antioxidant ability, Dragea, Gymnema, Tiliacora, leaves juices, Dry extract for animal useAbstract
The natural products for producing antioxidant under non chemical extraction are safe for using. The experiment designed to measure the antioxidant ability (DPPH-RSA) in juice extracts of 3 different local plants leaves; Pak Huen (Dragea), Pak Chiengda (Gymnema) and Pak Yanang (Tiliacora). Samples were collected from 4 different districts in Chiangmai province. The randomized complete block design consisted of 3 treatments and 4 blocks was used. The extraction started by chopping and grinding fresh leaves. The wet ground leaves were pressed in a cylinder with the hydraulic jack and collected the juices. The test solutions were measured with spectrophotometer after mixed with DPPH. As results, juice yield were 35.28, 33.88 and 32.28 percent of fresh leaves respectively, and dry extracts were 3.04, 2.90 and 2.30 percent respectively. The calculated RSA value showed significance result. Dragea had the highest RSA at 78.16% which was higher (p<0.05) than Gymnema (73.59%) and Tiliacora (47.18%). The difference between Gymnema and Tiliacora was also significant (p<0.05). Dry extracts can be prepared by using Cassava starch as the absorbant and dried at 60 °C in the oven. Dry extract of Dragea (Pak Huen) leaves had 6.62 mg/ml IC50, 4.49 % crude protein, and 3.19 Mcal/kg Metabolizable energy. It can be used as sources of antioxidant and energy in animal feed.
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