Optimization of protein extraction condition from soybean using pulse electric field
Keywords:
Soybean, Protein, Voltage, Specific energy consumption, Functional propertiesAbstract
The objective of this research was to determine protein extraction condition from soybean using pulse electric field. The study was divided into 3 parts. In the first part, the optimal extraction time at 10.5, 11.5 and 12.5 kV based on the highest protein content, percentage of yield and electrical conductivity disintegration index was determined. The second part, the effects of different voltages on protein content, percentage of yield, protein profile, functional properties and specific energy consumption were investigated. The final part, the effects of different soybean-to-water ratios on protein content, percentage of yield, protein profile and functional properties were performed. The results showed that extraction protein from soybean using pulse electric field at voltage of 12.5 kV for 60 min with soybean-to-water ratio of 1:15 w/v were suggested to be the optimum condition by considering the highest values of protein content, percentage of yield and functional properties. Protein profile also was revealed that beta-conglycinin (7S) protein affecting on functional properties were mostly found.
References
Yu X, Gouyo T, Grimi N, Bals O, Vorobiev E. Pulsed electric field pretreatment of rapeseed green biomass (stems) to enhance pressing and extractives recovery. Bioresour Technol. 2016;199:194-201.
Lopez N, Puertolas E, Condon S, Raso J. Enhancement of the extraction of betanine from red beetroot by pulsed electric fields. J Food Eng. 2009;90:60-66.
Ghosh S, Gillis A, Sheviryov J, Levkov K. Towards waste meat biorefinery: Extraction of proteins from waste chicken meat with non-thermal pulsed electric fields and mechanical pressing. J Clean Prod. 2018;208: 220-231.
Loginova K V, Lebovka N I, Vorobiev E. Pulsed electric field assisted aqueous extraction of colorants from red beet. J Food Eng. 2011;106:127-133.
Bradford M M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976;72(1-2):248-254.
Yousf N, Nazir F, Salim R, Ahsan H, Sirwal A. Water solubility index and water absorption index of extruded product from rice and carrot blend. J Pharmacog Phytochem. 2017;6(6):2165-2168.
Naowakul B, Wirjantoro T, Phianmongkhol A. Effects of speed and time of wet milling on properties of dietary fiber powder from pomelo's albedo. Food App Biosci J. 2013;1(1):34-48.
Chareemuy W, Tangduangdee C, Viriyarattanasak C. Dehulling effectiveness and properties of full–fat soy flour as affected by thermal treatments. J Agric Sci. 2020;49(1):96–112.
Polikovsky M, Fernand F, Sack M, Frey W, Müller G, Golberg A. Towards marine biorefineries: Selective proteins extractions from marine macroalgae Ulva with pulsed electric fields. Innov Food Sci Emerg Technol. 2016;37:194-200.
Goettel M, Eing C, Gusbeth C, Straessner R, Frey W. Pulsed electric field assisted extraction of intracellular valuables from microalgae. Algal Res. 2013;2(4):401-408.
Valle E D, Marracino P, Pakhomova O, Libert M, Apollonio F. Nanosecond pulsed electric signals can affect electrostatic environment of proteins below the threshold of conformational effects: The case study of SOD1 with a molecular simulation study. PLoS One. 2019;14(8):e0221685.
Hosain D, Hosainpour A, Farzad N, Mohammad-Hadi K. Ohmic processing: temperature dependent electrical conductivities of lemon juice. Modern App Sci. 2011;5(1):209-216.
Liu Z, Chang SKC, Li L, Tatsumi E. Effect of selective thermal denaturation of soybean proteins on soymilk viscosity and tofu's physical properties. Food Res Inter. 2004;37(8):815-822.
Redondo D, Venturini M E, Luengo E, Raso J, Arias E. Pulsed electric fields as a green technology for the extraction of bioactive compounds from thinned peach by-products. Innov Food Sci Emerg Technol. 2018;45:335-343.
Dehez F, Delemotte L, Kramar P, Miklavcic D, Tarek M. Evidence of conducting hydrophobic nanopores across membranes in response to an electric field. J Physic Chem C. 2014;118(13):6752-6757.
Chindapan R. Food foams. J Food Technol, Siam University. 2005;1(1):12-16.
Rattanapanone N. Basic principles of food processing, 1st ed. Bangkok: Odeon Store; 2001. 148 p.
Xu Y, Wang Z, Qi B, Ran A, Guo Z, Jiang L. Effect of oxidation on quality of Chiba tofu produced by soy isolate protein when subjected to storage. Foods. 2020; 9(12):1877.
He G, Yin Y, Yan X, Wang Y. Application of pulsed electric field for treatment of fish and seafood. In: Miklavcic D, editor. Handbook of electroporation, 1st ed. Zug: Springer International Publishing AG. 2017. p. 2637-2655.
He D, Zhang Z, Li H, Xia Y, Li X, Chen T. Optimizing functional properties of perilla protein isolate using the response surface methodology. Food Sci Technol. 2018;38(2):348-355.
Kanthamoon K, Varith J, Narkprasom K, Tanongkankit Y. Application of pulsed electric field for extraction of soy protein. Proceeding of the 12th International Conference on Science, Technology and Innovation for Sustainable Well-Being (STISW XII); 2020 Jun 26-28; Phetchaburi, Thailand. Silpakorn University; 2020.
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