Protective effects of Nelumbo nucifera extract on dermal fibroblast against hydrogen peroxide-induced cell toxicity
Keywords:
Nelumbo nucifera, Fibroblast, Hydrogen peroxide, Polyphenol, AntioxidantAbstract
Dermal fibroblasts play a key role in maintaining skin homeostasis by synthesizing and degrading extracellular matrix components. Oxidative stress induced by reactive oxygen species is harmful to the cells, resulting in cellular senescence and premature cell death. The present research work was aimed to evaluate whether Nelumbo nucifera petal extract prevents oxidative stress-induced cell death. Fibroblast cells were exposed to various concentrations of N. nucifera extract (0-1,000 µg/ml) prior to hydrogen peroxide (H2O2) treatment (400 µM). The extract (400-1,000 µg/ml) could increase cell viability significantly after 48-hr H2O2 exposure, while the extract itself was not toxic. Furthermore, the extract was rich in flavonoid and polyphenol content, 26.39±1.94 mgQE/g and 39.26±1.56 mgGAE/g, respectively. That confirmed the in vitro antiradical activity against DPPH and ferric-reducing power. The antioxidant activity of the extract might be plausible for preventing hydrogen peroxide-induced cell death in dermal fibroblasts. Further study should consider the potential of N. nucifera in fibroblast senescence model for anti-aging effect of it.
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