The effects of sucrose, activated charcoal, and coconut dust on in vitro corm induction of Gladiolus hybrida under aseptic technique

Authors

  • Sukalya Poothong Agriculture, School of Agriculture and Natural Resources, University of Phayao
  • Nutchanan Duangkon Agriculture, School of Agriculture and Natural Resources, University of Phayao
  • Suwara Sukanthamala Agriculture, School of Agriculture and Natural Resources, University of Phayao

Keywords:

Sucrose, Activated charcoal, Coconut dust, Gladiolus hybrida

Abstract

This study aimed to investigate the effect of sucrose concentrations: of 3%, 6%, and 9% together with or without two different supplementary matters: activated charcoal (0.01%) and coconut dust (10%) on in vitro growth and corm induction of Gladiolus hybrida when cultured for 5 weeks. The objective was to investigate the optimal culture media for corm production in long-term culture. The results showed that shoots grown on MS medium with the addition of 10% coconut dust at any sucrose concentrations had a high percentage of corm induction, ranging from 72 to 88. The treatment of 6% sucrose supplemented with 0.01% activated charcoal and 10% coconut dust yielded the highest corm induction percentage (88%) after being cultured for 5 months. Therefore, the optimum medium for corm induction of Gladiolus hybrida was MS medium supplemented with 6% sucrose, 0.01% activated charcoal, and 10% coconut dust.

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Growth appearance of gladiolus shoots after being cultured in different medium conditions for 5 weeks. (Bar I = 1 cm)

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Published

2023-08-26

How to Cite

1.
Poothong S, Duangkon N, Sukanthamala S. The effects of sucrose, activated charcoal, and coconut dust on in vitro corm induction of Gladiolus hybrida under aseptic technique . Health Sci Tech Rev [Internet]. 2023 Aug. 26 [cited 2024 May 17];16(2):28-39. Available from: https://li01.tci-thaijo.org/index.php/journalup/article/view/256469

Issue

Vol. 16 No. 2 (2023): May - August

Section

Research articles

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