The inhibitory effect of Pogonatherum paniceum extracts on HT29 colorectal cancer cells growth

Authors

  • Benyalak Moomla Division of Microbiology, School of Medical Sciences, University of Phayao
  • Saridapha Boonsrikun Division of Microbiology, School of Medical Sciences, University of Phayao
  • Sinittra Thakaew Division of Microbiology, School of Medical Sciences, University of Phayao
  • Anchalee Rawangkan Division of Microbiology, School of Medical Sciences, University of Phayao
  • Grissana Pook-In Division of Microbiology, School of Medical Sciences, University of Phayao
  • Rungthip Thongboontho Division of Biochemistry, School of Medical Sciences, University of Phayao
  • Thatsani Sripho Department of Emergency Medical Operation, School of Medicine, University of Phayao
  • Ratsada Peaphasawat Department of Pathology, School of Medicine, University of Phayao

Keywords:

Colorectal cancer, Anticancer, Apoptosis, Pogonatherum paniceum extracts

Abstract

Colorectal cancer is the third most frequent cancer worldwide. It is more common in adults over the age of 50, while environmental and genetic variables have a part to play in etiology. However, studies have indicated that nutrition plays a role in both the cause and prevention of colon cancer. Pogonatherum paniceum (Lam.) Hack (PP) is a grass family with exceptional detoxification properties. This study is focused on the anti-proliferative effect of PP extract and apoptosis related mechanism. MTT, MTS, and colony formation assays were used to evaluate the anti-proliferative activities of human HT29 colon cancer cells. The results suggest that greater concentrations of PP extract reduced the viability of HT29 cancer cells after 48 hours, as shown by an IC50 of 1.2 ± 0.06 mg/mL. Moreover, PP extract induces apoptosis in HT29 cancer cells in a dose-dependent manner (0.5-0.75 mg/mL). In conclusion, PP extract is an alternative treatment for colorectal cancer cells.

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การทดสอบการยับยั้งการเจริญของเซลล์ HT29 ด้วยสารสกัดไผ่จืด (A) แสดงขนาดและจำนวนโคโลนี, (B) แสดงจำนวนโคโลนี ที่ความเข้มข้นต่างๆ ที่เวลา 48 ชั่วโมง (**p<0.01 แสดงความแตกต่างอย่างมีนัยสำคัญทางสถิติ) (สเกลบาร์ = 50 µm)

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Published

2024-04-09

How to Cite

1.
Moomla B, Boonsrikun S, Thakaew S, Rawangkan A, Pook-In G, Thongboontho R, Sripho T, Peaphasawat R. The inhibitory effect of Pogonatherum paniceum extracts on HT29 colorectal cancer cells growth. Health Sci Tech Rev [Internet]. 2024 Apr. 9 [cited 2024 May 19];17(1):15-23. Available from: https://li01.tci-thaijo.org/index.php/journalup/article/view/258558

Issue

Vol. 17 No. 1 (2024): January - April 2024

Section

Research articles

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