Effect of Glyphosate on Mycelial Growth, Spore Germination and Indoleacetic acid-Producing of Endophytic Fungi Isolated from Thunbergia laurifolia Linn.
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The effect of glyphosate on in vitro mycelium growth, spore germination and Indoleacetic acid production (IAA) of endophytic fungi isolated from Thunbergia laurifolia Linn. Isolated endophytic fungi were 10 isolate but only 5 isolates could produce IAA and these endophytic fungi are classified by internal transcribed spacer (ITS) analysis to be Corynespora cassiicola and Colletotrichum gloeosporioides. Mycelium and spore growth were tested on Potato Dextrose Agar (PDA) medium containing 10, 20, 30, 40 and 30 mM glyphosate for 7 days. The Mycelium and spore growth were more inhibited when the concentration of glyphosate increased. Mycelium of C. cassiicola could grow at 20 and 10 mM glyphosate (92.32 and 87.97% of control, respectively) and C. gloeosporioides could grow at 30 and 20 mM glyphosate (77.80 และ 76.15% of control, respectively). Spore germination could germinate at 10 mM glyphosate (78.55 and 63.67% of control) in both fungi. The IAA production of each species were tested in PDB containing 2 mg/l tryptophan and varying concentration of glyphosate for 12 days. The result showed that increasing of glyphosate concentration decreased IAA production of both fungi. C. gloeosporioides and C. cassiicola could produce the highest IAA concentration in PDB containing glyphosate at 10 mM glyphosate (125 and 51.73 mg/l that were 64.1 and 52.4% of control, respectively)
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