The isolation of cellulase producing bacteria from paddy field soil samples in Phra Nakhon Si Ayutthaya province
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Abstract
The objective of this research was to isolate cellulase producing bacteria from rice paddy soil samples in Phra Nakhon Si Ayutthaya province. Soil samples of rice fields in five districts, namely Bangzai, Bangban, Tharuea, Phachi and Wangnoi, were used to screen for cellulase producing bacteria by serial ten-fold dilution method on cellulose-degrading culture medium. The bacterial colonization showed in the range of 1.06-6.87×106 CFU/g, and twenty-six isolates of bacteria were isolated. Morphological characterization of most isolates was gram-positive rod-shaped bacteria (80.77%). The results of cellulase production test on carboxymethyl cellulose agar were found that only four isolates (15.38%) produced cellulase, which a halo zone around the colony larger than the zone produced by positive control bacteria, Paenibacillus curdlanolyticus strain B-6. The isolate PC-C-1 had the highest halo zone size (2.56±1.03 centimeters). Moreover, the rice straw degradation test of isolate PC-C-1 in nutrient broth added 5% rice straw at 1×108 cell/mL showed digestion of rice straw up to 64.80%. These results can be used as preliminary data for further studies of the practical application in rice straw degradation by bacteria, which is an agricultural waste reduction in the future.
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Published manuscript are the rights of their original owners and RMUTSB Academic Journal. The manuscript content belongs to the authors' idea, it is not the opinion of the journal's committee and not the responsibility of Rajamangala University of Technology Suvarnabhumi
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