Effects of culture medium, benzylaminopurine and temporary immersion bioreactor system on growth of chrysanthemum under aseptic condition

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Irada Thinsri
Paweena Pumisutapon

Abstract

          In this research, the factors affecting on growth of chrysanthemum (WG) cultured under aseptic condition were studied. The single nodal explants were cultured on a semi-solid medium including ¼ MS, ½ MS and MS added with cytokinin 6-benzylaminopurine (BAP) at 0, 0.25, 0.50, and 1.00 mg/L for a period of 8 weeks. It was found that all treatments produced only 1 new shoot per explant, and number of nodes per shoot were not statistically different among treatments. The ½ MS medium without growth regulator showed the highest shoot length, number of nodes per shoot and shoot fresh weight at 7.36 cm, 7.40 nodes, and 333.07 mg, respectively. Furthermore, the effect of different culturing systems was compared. The single nodal explants were cultured using ½ MS medium without a growth regulator combined with the twin-flasks temporary immersion bioreactor (TIB) system with liquid medium feeding every 24 h for 4, 5, and 6 min each time, and compared with the semi-solid medium. The results showed that after a period of 8 weeks, the feeding liquid medium every 24 h for 5 min each time was suitable for cultivation. This feeding condition gave the highest number of shoots per explant, number of nodes per shoot, and shoot fresh weight at 1.48 shoots, 10.10 nodes, and 841.67 mg, respectively, which were increased 1.40, 1.26 and 2.53 times, respectively compared to those of semi-solid medium. However, the hyperhydricity (50%) was found as abnormal characteristic when liquid medium was fed every 24 h for 6 min each time.

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บทความวิจัย (Research article)

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