Evaluation of fungicide carbendazim resistance in Colletotrichum gloeosporioides causing anthracnose disease of mango cv. Aokrong in Chanthaburi province
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Abstract
Colletotrichum gloeosporioides, the causal agent of ‘Aokrong’ mango anthracnose disease was isolated by using tissue transplanting technique from infected fruits of orchards in Chanthaburi province. Twenty-seven isolates of fungi were obtained. The carbendazim resistance assays were conducted by growing all isolates on potato dextrose agar (PDA) containing the carbendazim at various concentrations 0.1, 1, 10, 100, 500 and 1,000 ppm. The fungal resistance to carbendazim was evaluated and classified. The results revealed that 14 isolates (51.85%) were classified as HR (highly resistance) phenotype and 13 isolates (48.15%) were S (sensitive) phenotype. None of the isolates were classified as MR (moderate resistance) or WR (weakly resistance) phenotype. The effectiveness of 9 fungicides at recommended concentrations to inhibit mycelial growth of HR phenotype C. gloeosporioides was tested using poisoned food technique. The results showed the reduction of mycelia growth of all tested fungicides to the fungi as compared to untreated control. Mancozeb, captan, prochloraz, difenoconazole+azoxystrobin and difeconazole+propiconazole showed 100% inhibition of mycelium growth of C. gloeosporioides, followed by carboxin, benomyl, carbendazim and copper hydroxide inhibited mycelium growth by 87.78, 72.22, 64.89 and 22.89%, respectively. This study provides preliminary information on the types of fungicide that are suitable for managing anthracnose of mango fruits in Chanthaburi province.
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