Flocculation efficiency of microalgae Selenastrum bibraianum BPR1107 by flocculants

Main Article Content

Waratit Donsujit
Narumon Boonkrachang
Thaithaworn Lirdwitayaprasit

Abstract

          Microalgae biomass can be harvested in several methods for oil extraction and biodiesel production. This study was investigated the green microalgae Selenastrum bibraianum BPR1107 using chemicals to achieve coagulation and flocculation processes. Three chemical flocculants (i.e., aluminium sulphate, ferric chloride and polyaluminium chloride) were evaluated for their flocculation efficiency. The results were showed that polyaluminium chloride provided a maximum flocculation efficiency of 99.87±0.12 percent at 0.21 g/L in sedimental time of 60 minutes. The different initial concentration of microalgae culture affected the optimum flocculant dose and flocculation efficiency. In addition, excessive or less use of flocculant dose resulted in a decrease of flocculation efficiency. Therefore, the correlation between each dose of flocculants and OD680 expressed by optimal dose of polyaluminium chloride (g/L)=0.4202OD680-0.0004 (R2=1). The optimal dose was estimated from this function, indicating the flocculation efficiency was more than 99 percent in all studied concentrations of microalgae culture.

Article Details

How to Cite
Donsujit, W., Boonkrachang, N., & Lirdwitayaprasit, T. (2021). Flocculation efficiency of microalgae Selenastrum bibraianum BPR1107 by flocculants. RMUTSB ACADEMIC JOURNAL, 9(2), 133–149. Retrieved from https://li01.tci-thaijo.org/index.php/rmutsb-sci/article/view/250998
Section
Research Article

References

Ahmad, A. L., Yasin, N. H. M., Derek, C. J. C., & Lim J. K. (2011). Optimization of microalgae coagulation process using chitosan. Chemical Engineering Journal, 173, 879-882.

Aramaki, T., Watanabea, M. M., Nakajimaa, M., & Ichikawa, S. (2020). Bench-scale dehydration of a native microalgae culture by centrifugation, flocculation and filtration in Minamisoma city, Fukushima, Japan. Bioresource Technology Reports, 10, 100414.

Barros, A. I., Gonçalves, A. L., Simões, M., & Pires, J. C. M. (2015). Harvesting techniques applied to microalgae: A review. Renewable and Sustainable Energy Reviews, 41, 1489-1500.

Chakravarty, S., & Mallick, N. (2019). Optimization of lipid accumulation in an aboriginal green microalga Selenastrum sp. GA66 for biodiesel production. Biomass and Bioenergy, 126, 1-13.

Chen, L., Wang, C., Wanga, W., & Wei, J. (2013). Optimal conditions of different Flocculation methods for harvesting Scenedesmus sp. cultivated in an open-pond system. Bioresource Technology, 133, 9-15.

Chisti, Y. (2007). Biodiesel from microalgae. Biotechnology, 25, 294-306.

de Godos, I., Guzman, H. O., Soto, R., Garcia-Encina, P. A., Becares, E., Munoz, R., & Vargas, V. (2011). Coagulation/flocculation-Based removal of algal-bacterial biomass from piggery wastewater treatment. Bioresource Technology, 102(2), 923-927.

Donsujit, W., Khonsue, T., & Lirdwitayaprasit, T. (2020). Growth and fatty acid composition of microalgae Selenastrum bibraianum BPR1107 isolated from Bangpra reservoir, Chonburi province, under laboratory and outdoor condition cultivation. Burapha Science Journal, 25(3), 1052-1066. (in Thai)

EPPO. (2014). Biodiesel. Retrieved August 5, 2021, from http://www.eppo.go.th/images/Infromation_service/Publication/Knowledge/biodiesel.pdf

Gerchman, Y., Vasker, B., Tavasi, M., Mishael, Y., Kinel-Tahan, Y., & Yehoshua, Y. (2017). Effective harvesting of microalgae: Comparison of different polymeric flocculants. Bioresource Technology, 228, 141-146.

Goh, B. H. H., Ong, H. C., Cheah, M. Y., Chen, W. H., Yu, K. L., & Mahlia, T. M. I. (2019). Sustainability of direct biodiesel synthesis from microalgae biomass: a critical review. Renewable and Sustainable Energy Reviews, 107, 59-74.

Gonzalez-Fernandez, C., & Munoz, R. (2017). Microalgae-based biofuels and bioproducts: From feedstock cultivation to end-products. Duxford: Woodhead.

Gorin, K. V., Sergeeva, Y. E., Butylin, V. V., Komova, A. V., Pojidaev, V. M., Badranova, G. U., Shapovalova, A. A., Konova, I. A., & Gotovtsev, P. M. (2015). Methods coagulation/flocculation and flocculation with ballast agent for effective harvesting of microalgae. Bioresource Technology, 193, 178-184.

Grima, E. M., Belarbi, E.–H., Acien Fernandez, F. G., Medina, A. R., & Chisti, Y. (2003). Recovery of microalgal biomass and metabolites: process options and economics. Biotechnology Advances, 20, 491-515.

Jacob-Lopes, E., Cacia, L. M., Lacerda, F., & Franco, T. T. (2008). Biomass production and carbon dioxide fixation by Aphanothece microscopica Nägeli in a bubble column photobioreactor. Biochemical Engineering Journal, 40, 27-34.

Kim, D.-G., La, H.-J., Ahn, C.-Y., Park, Y.-H., & Oh, H.-M. (2011). Harvest of Scenedesmus sp. with bioflocculant and reuse of culture medium for subsequent high-density cultures. Bioresource Technology, 102, 3163-3168.

Li, S., Hu, T., Xu, Y., Wang, J., Chu, R., Yin, Z., Mo, F., & Zhu, L. (2020). A review on flocculation as an efficient method to harvest energy microalgae: Mechanisms, performances, influencing factors and perspectives. Renewable and Sustainable Energy Reviews, 131, 1100-1115.

Mata, T. M., Martins, A. A., & Caetano, N. S. (2010). Microalgae for biodiesel production and other applications: A review. Renewable and Sustainable Energy Reviews, 14, 217-232.

Mathimani, T., & Mallick, N. (2018). A comprehensive review on harvesting of microalgae for biodiesel – key challenges and future directions. Renewable and Sustainable Energy Reviews, 9, 1103-1120.

Mori, C. C., Bagatini, I. L., da Silva, T. G., Parrish, C. C., & Vieira, A. A. H. (2018). Use of fatty acids in the chemotaxonomy of the family Selenastraceae (Sphaeropleales, Chlorophyceae). Phytochemistry, 151, 9-16.

Nimusornsakul, A. (1995). Reuse of treated water from a bio-rotating disc system through a chemical process with polyaluminum chloride (Master's thesis). Kasetsart University, Bangkok. (in Thai)

Ogbonna, C. N., & Nwoba, E. G. (2021). Bio-based flocculants for sustainable harvesting of microalgae for biofuel production. A review. Renewable and Sustainable Energy Reviews, 139, 110690.

Oilgae. (2013). Comprehensive oilgae report, energy from algae: Products, market, processes & strategies. Tamilnadu: Oilgae.

Okoro, V., Azimova, U., Munozb, J., Hernandezc, H. H., & Phand, A. N. (2019). Microalgae cultivation and harvesting: Growth performance and use of flocculants - A review. Renewable and Sustainable Energy Reviews, 115, 109364.

Papazi, A., Markridis, P., & Divanach, P. (2010). Harvesting Chlorella minutissima using cell coagulants. Journal of Applied Phycology, 22, 349-355.

Pernitsky, D. J., & Edzwald, J. K., (2006). Selection of alum and polyaluminum coagulants: principles and applications. Journal of Water Supply: Research and Technology-Aqua, 55(2), 121-141.

Reyes, J. F., & Labra, C. (2016). Biomass harvesting and concentration of microalgae scenedesmus sp. cultivated in a pilot photobioreactor. Biomass and Bioenergy, 87, 78-83.

Richmond, A., & Becker, E. W. (1986). Technological aspects of mass cultivation-a general outline. In A. Richmond (Ed.), CRC Handbook of microalgae mass culture (pp. 245-263). Boca Raton: CRC Press.

Rocha, G. S., Parrish, C. C., Lombardi, A. T., & Melão, M. da G. G. (2018). Biochemical and physiological responses of Selenastrum gracile (Chlorophyceae) acclimated to different phosphorus concentrations. Journal of Applied Phycology, 30, 2167-2177.

Shelef, G., Sukenik, A., & Green, M. (1988). Microalgae harvesting and processing: A literature review SERI/STR-231-2396, UC Category, 61a, DE84013036 (Subcontract report). Haifa, Israel: Technion Research and Development Foundation.

Srimoon, R., Darapong, P., & Kanavong, V. (2014). Extraction of Alexandrian Laurel (Calophyllum inophyllum L.) seed oil for biodiesel production. RMUTSB Academic Journal, 2(2), 131-138. (in Thai)

Stanier, R. Y., Kunisawa, R., Mandel, M., & Cohen-Bazire, B. G. (1971). Purification and properties of unicellular blue green algae (Order Chroococcales). Bacteriology Review, 35(2), 171-205.

Sudbundit, A., & Changchai, N. (2018). The paint factory’s wastewater: COD removal by chemical precipitation method. RMUTSB Academic Journal, 6(2), 171-181. (in Thai)

Tanthulwet, M. (1996). Water supply engineering, Volume 1 (3rd ed.). Bangkok: Chulalongkorn University Press. (in Thai)

Uduman, N., Ying, Q., Danquah, M. K., Forde, G. M., & Hoadley, A., (2010). Dewatering of microalgae cultures: A major bottleneck to algae-based fuel. Renewable and Sustainable Energy, 2, 012701/1 – 012701/15.

Vandamme, D., Foubert, I., Meesschaert, B., & Muylaert, K. (2010). Flocculation of microalgae using cationic starch. Journal of Applied Phycology, 22, 525-530.

Wang, Q., Oshita, K., & Takaoka, M. (2021). Flocculation properties of eight microalgae induced by aluminum chloride, chitosan, amphoteric polyacrylamide, and alkaline: Life-cycle assessment for screening species and harvesting methods. Algal Research, 54, 102226.

Watcharenwong, A., & Bailuang, Y. (2017). Using of organic coagulant with magnetic coagulant in raw water suspended particle removal for water supply system (research report). Nakhon Ratchasima: Suranaree University of Technology. (in Thai)

Wu, Z., Zhu, Y., Huang, W., Zhang, C., Li, T., Zhang, Y., & Li, A. (2012). Evaluation of flocculation induced by pH increase for harvesting microalgae and reuse of flocculated medium. Bioresource Technology, 12, 234-241.

Wu, J., Liu, J., Lin, L, Zhang, C., Li, A., Zhu, Y., & Zhang, Y. (2015). Evaluation of several flocculants for flocculating microalgae. Bioresource Technology, 197, 495-501.

Xiao, M., Shin, H-J., & Dong, Q. (2013). Advances in cultivation and processing techniques for microalgal biodiesel: a review. Korea Journal of Chemical Engineering, 30(12), 2119-2126.

Yousuf, A. (2020). Microalgae Cultivation for Biofuels Production. London: Academic Press.

Zhu, L., Nugroho, Y. K., Shakeel, S. R., Li, Z, Martinkauppi, B., & Hiltunen, E. (2017). Using microalgae to produce liquid transportation biodiesel: what is next?. Renewable and Sustainable Energy Reviews, 78, 391-400.

Zhu, L., Pan, G., Xu, H., Kong, L., Guo, W., Yu, J., Mortimer, R. J. G., & Shi, W. (2021). Enhanced chitosan flocculation for microalgae harvesting using electrolysis. Algal Research, 55, 102268.