Effect of carbon and nitrogen sources on antibacterial substance production from isolated Actinomycetes
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Abstract
Actinomycetes are Gram’s positive bacteria that has the best ability to produce a bioactive compound that inhibited other bacterial growth. This research aimed to isolate, select and study the effect of carbon and nitrogen sources on anti-pathogenic bacterial substances from isolated actinomycetes. Actinomycetes were collected from archaeological site surface in Phranakhon Si Ayutthaya Province. Actinomycetes isolation was isolated using Starch Casein Agar (SCA). The anti-pathogenic bacterial activity was done against 5 pathogenic bacterial strains including Bacillus cereus TISTR 2372, Escherichia coli TISTR 527, Klebsiella pneumonia TISTR 1867, Pseudomonas aeruginosa TISTR 2370 and Staphylococcus aureus TISTR 746 using agar disc diffusion method. The results showed that isolate 1IJ04 had the highest activity on growth inhibition of B. cereus TISTR 2372 with a diameter of inhibition zone of 17 mm. The optimal carbon and nitrogen sources for growth of isolate 1IJ04 were casava starch and monosodium glutamate. This study is the basis study of the diversity of actinomycetes that produce bioactive compounds. This could be benefit for the next antibiotic development.
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