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The study of auxin production and nitrogen fixation by azotobacter

The study of auxin production and nitrogen fixation by azotobacter was conducted by soil composite sampling of 15 cm. of deep soil from plantation area in Phra Nakhon Si Ayutthaya province (16 samples). The efficiency of 16 isolates was tested using Completely Randomized Design (CRD) with four replications on nitrogen fixation by acetylene reduction. The best ten isolates of nitrogen fixing as A1, A2, A3, A4, A5, A6, A7, A8, A9 and A10, which fixed nitrogen 3 days after incubation gave 0.94, 0.98, 0.98, 0.98, 0.97, 0.96, 0.98, 0.99, 0.96 and 0.96 mg N/hr respectively. Three isolates A10, A4 and A8 of auxin production showed (35.00 20.48 and 19.76 μg/ml) respectively.