Effect of Extraction Methods on Total Phenolic Content, Antioxidant Activity and Antibacterial Activity of Ethanolic Leaf Extracts of Kratom (Mitragyna speciosa)
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Abstract
This research aimed to examine the effect of extraction methods on total phenolic contents, antioxidant activity and antibacterial activity of ethanolic leaf extracts of kratom (Mitragyna speciosa) prepared by 2 extraction methods including maceration and ultrasonic extraction. Total phenolic content analysis using the Folin-Ciocalteu method revealed that total phenolic contents of the extracts prepared by maceration and ultrasonic extraction were 2.51±0.03 and 2.89±0.03 mg gallic acid equivalent/g dry extract, respectively. Antioxidant activity analysis using DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging assay demonstrated that half maximal inhibitory concentrations (IC50) of free radical DPPH of the extracts prepared by maceration and ultrasonic extraction were 15.60±0.25 and 15.56±0.18 µg/mL, respectively. Antibacterial activity analysis using disc diffusion method and broth microdilution method revealed that the extracts from both extraction methods inhibited Bacillus subtilis, Staphylococcus aureus and Staphylococcus epidermidis but not Escherichia coli. This research demonstrated that both extraction methods, maceration and ultrasonic extraction, did not have statistically significant effect on total phenolic contents, antioxidant activity and antibacterial activity of ethanolic leaf extracts of kratom.
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บทความที่ได้รับการตีพิมพ์เป็นลิขสิทธิ์ของ วารสารวิทยาศาสตร์และเทคโนโลยี มหาวิทยาลัยอุบลราชธานี
ข้อความที่ปรากฏในบทความแต่ละเรื่องในวารสารวิชาการเล่มนี้เป็นความคิดเห็นส่วนตัวของผู้เขียนแต่ละท่านไม่เกี่ยวข้องกับมหาวิทยาลัยอุบลราชธานี และคณาจารย์ท่านอื่นๆในมหาวิทยาลัยฯ แต่อย่างใด ความรับผิดชอบองค์ประกอบทั้งหมดของบทความแต่ละเรื่องเป็นของผู้เขียนแต่ละท่าน หากมีความผิดพลาดใดๆ ผู้เขียนแต่ละท่านจะรับผิดชอบบทความของตนเองแต่ผู้เดียว
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