Development of Antioxidant and Anti-inflammatory Gels Containing the Extract
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Abstract
This research aimed to develop antioxidant and anti-inflammatory gels containing the extract of mixed Syzygium aromaticum flower buds and Myristica fragrans seeds. The preparation of herbal ethanol extracts was performed by maceration method using 95% ethanol as a solvent. The antioxidant activity of ethanol extract of S. aromaticum flower buds (SAFB), ethanol extract of M. fragrans seeds (MFS) and ethanol extract of mixed herbs consisting of an equal ratio of SAFB and MFS was assessed by ABTS and DPPH assays. The anti-inflammatory activity of the extracts was assessed via inhibition of nitric oxide (NO) production using Griess method. The results revealed that the extract of mixed SAFB and MFS exhibited high ABTS•+ and DPPH• radical scavenging activities with IC50 values of 0.35±0.01 and 0.08±0.01 mg/mL, respectively, and was able to inhibit NO production with IC50 value of 0.19±0.01 mg/mL. After the extract of mixed SAFB and MFS was used to develop gel formulations containing 0.05%, 0.10%. and 0.20% w/w of the extract, the herbal gels were subjected to the accelerated stability test using heat-cool cycling method and the assessment of antioxidant and anti-inflammatory activities using the aforementioned methods. The results demonstrated that all herbal gel formulations showed the good stability with no change in physical and chemical characteristics. Moreover, all herbal gel formulations possessed antioxidant and anti-inflammatory activities comparable to the standard gel, 1% diclofenac gel. Therefore, all gels containing the extract of mixed SAFB and MFS used in this study have potential for further development as herbal products with antioxidant and anti-inflammatory activities.
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บทความที่ได้รับการตีพิมพ์เป็นลิขสิทธิ์ของ วารสารวิทยาศาสตร์และเทคโนโลยี มหาวิทยาลัยอุบลราชธานี
ข้อความที่ปรากฏในบทความแต่ละเรื่องในวารสารวิชาการเล่มนี้เป็นความคิดเห็นส่วนตัวของผู้เขียนแต่ละท่านไม่เกี่ยวข้องกับมหาวิทยาลัยอุบลราชธานี และคณาจารย์ท่านอื่นๆในมหาวิทยาลัยฯ แต่อย่างใด ความรับผิดชอบองค์ประกอบทั้งหมดของบทความแต่ละเรื่องเป็นของผู้เขียนแต่ละท่าน หากมีความผิดพลาดใดๆ ผู้เขียนแต่ละท่านจะรับผิดชอบบทความของตนเองแต่ผู้เดียว
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