Biodiversity and Potential for Producing Biological Substances of Actinomycetes Isolated from Soil in Phanom Rung Historical Park, Buriram Province
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Abstract
Actinomycetes are bacteria having the well-known ability to produce biological substances with a variety of properties such as pigments, antimicrobials and antioxidants. This research aimed to isolate, select and study the ability to produce some biological substances of actinomycetes isolated from soil of Phanom Rung Histological Park, Buriram Province. The isolation of actinomycetes was conducted by spread plate technique on media suitable for growth of actinomycetes including Starch Casein Agar (SCA), International Streptomyces Project 1 (ISP1), Actinomyces Isolation agar (AIA) and Hickey’s Terner agar (HTA). Colony morphology of the isolated actinomycetes grown on SCA was studied under a stereo microscope. The ability to secret pigments out of the cells was examined in Starch Casein Broth (SCB). Antibacterial ability was tested by using Agar block method against five species of pathogenic bacteria including Escherichia coli TISTR 527, Klebsiella pneumoniae TISTR 1867 Pseudomonas aeruginosa TISTR 2370 Bacillus cereus TISTR 2372 and Staphylococcus aureus TISTR 746 by using Agar block method. Furthermore, the effects of temperature and carbon sources in culture media on ability of actinomycetes to produce pigments and antibacterial substances were also investigated. From the results, 123 isolates of actinomycetes were isolated. They were grouped into 8 and 4 groups according to their colony morphologies and ability to produce antibacterial substances, respectively. In addition, temperature and carbon sources in culture media were found to affect ability of actinomycetes to produce pigments and antibacterial substances.
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บทความที่ได้รับการตีพิมพ์เป็นลิขสิทธิ์ของ วารสารวิทยาศาสตร์และเทคโนโลยี มหาวิทยาลัยอุบลราชธานี
ข้อความที่ปรากฏในบทความแต่ละเรื่องในวารสารวิชาการเล่มนี้เป็นความคิดเห็นส่วนตัวของผู้เขียนแต่ละท่านไม่เกี่ยวข้องกับมหาวิทยาลัยอุบลราชธานี และคณาจารย์ท่านอื่นๆในมหาวิทยาลัยฯ แต่อย่างใด ความรับผิดชอบองค์ประกอบทั้งหมดของบทความแต่ละเรื่องเป็นของผู้เขียนแต่ละท่าน หากมีความผิดพลาดใดๆ ผู้เขียนแต่ละท่านจะรับผิดชอบบทความของตนเองแต่ผู้เดียว
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