Effects of Sucrose on Microchirita purpurea D.J.Middleton & Tribon Germplasm Preservation by in vitro Minimal Growth Technique
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Abstract
This research aimed to study the effect of sucrose on Microchirita purpurea D.J.Middleton & Tribon germplasm preservation by in vitro minimal growth technique. The experiment was conducted using a completely randomized design with 6 treatments, 4 replicates per treatment and 2 explants per replicate. The 6 treatments were M. purpurea shoot explants grown on Murashige & Skoog (MS) medium supplemented with 6 different sucrose concentrations of including 0, 2, 4, 6, 8 and 10%. After growth for 12 weeks, it was found that the 10% sucrose supplementation could slow down the growth of M. purpurea resulting in the lowest average plant height (2.05 cm). This could prolong the subculture time for more than 3 months. Furthermore, it was also found that sucrose concentrations in MS medium affected average plant height and average leaf number but not average diameter of stem. This study demonstrated that sucrose concentrations in MS medium influenced M. purpurea germplasm preservation by in vitro minimal growth technique.
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บทความที่ได้รับการตีพิมพ์เป็นลิขสิทธิ์ของ วารสารวิทยาศาสตร์และเทคโนโลยี มหาวิทยาลัยอุบลราชธานี
ข้อความที่ปรากฏในบทความแต่ละเรื่องในวารสารวิชาการเล่มนี้เป็นความคิดเห็นส่วนตัวของผู้เขียนแต่ละท่านไม่เกี่ยวข้องกับมหาวิทยาลัยอุบลราชธานี และคณาจารย์ท่านอื่นๆในมหาวิทยาลัยฯ แต่อย่างใด ความรับผิดชอบองค์ประกอบทั้งหมดของบทความแต่ละเรื่องเป็นของผู้เขียนแต่ละท่าน หากมีความผิดพลาดใดๆ ผู้เขียนแต่ละท่านจะรับผิดชอบบทความของตนเองแต่ผู้เดียว
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