Total Phenolic Content, Total Flavonoid Content, Antioxidant and Anti-inflammatory Activities of Extracts of Herbs in Trisarn Recipe
Article Sidebar
Main Article Content
Abstract
Trisarn recipe is an herbal recipe consisting of 3 herbs including Plumbago indica L. root, Piper sarmentosum Roxb. root and Piper ribesioides Wall. Stem. It is commonly used as an adaptogen in the winter season. This research aimed to examine total phenolic content, total flavonoid content, antioxidant and anti-inflammatory activities of extracts of herbs in Trisarn recipe. Trisarn recipe extract and extracts of individual herbal component of Trisarn recipe were prepared by using 95% ethanol as a solvent. Antioxidant activity of the extracts was examined using DPPH assay, ABTS assay and FRAP assay. Total phenolic content and total flavonoid content of the extracts were analyzed by Folin-Ciocalteu method and aluminium chloride colorimetric assay, respectively. Anti-inflammatory activity of the extracts was conducted by assessing the ability of the extracts to inhibit the release of nitric oxide of lipopolysaccharide-stimulated RAW 264.7 macrophage cells. The results revealed that P. indica root extract exhibited the highest antioxidant activity. It IC50 values from DPPH assay and ABTS assay were 24.02 ± 1.72 µg/mL and 50.58 ± 5.59 µg/mL, respectively and its FRAP value was 568.43 ± 21.62 mM FeSO4 equivalent/g extract. Furthermore, it was found that P. indica root extract had the highest total phenolic content and total flavonoid content of 586.72 ± 17.14 mg gallic acid equivalent/g extract and 109.35 ± 4.54 mg quercetin equivalent/g extract, respectively. For anti-inflammatory activity examination, it was found that P. indica root extract exhibited the most potent inhibitory effect on the release of nitric acid with the IC50 value of 35.78 ± 1.58 µg/mL. This study demonstrates that P. indica root extract is a promising antioxidant and anti-inflammatory agent that may be of potential use.
Article Details
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
บทความที่ได้รับการตีพิมพ์เป็นลิขสิทธิ์ของ วารสารวิทยาศาสตร์และเทคโนโลยี มหาวิทยาลัยอุบลราชธานี
ข้อความที่ปรากฏในบทความแต่ละเรื่องในวารสารวิชาการเล่มนี้เป็นความคิดเห็นส่วนตัวของผู้เขียนแต่ละท่านไม่เกี่ยวข้องกับมหาวิทยาลัยอุบลราชธานี และคณาจารย์ท่านอื่นๆในมหาวิทยาลัยฯ แต่อย่างใด ความรับผิดชอบองค์ประกอบทั้งหมดของบทความแต่ละเรื่องเป็นของผู้เขียนแต่ละท่าน หากมีความผิดพลาดใดๆ ผู้เขียนแต่ละท่านจะรับผิดชอบบทความของตนเองแต่ผู้เดียว
References
Nair, S.V. and et al. 2016. Antimicrobial activity of plumbagin, a naturally occurring naphthoquinone from Plumbago rosea, against Staphylococcus aureus and Candida albicans. International Journal of Medical Microbiology. 306(4): 237-248.
Roy, A. 2021. Plumbagin: A potential anti-cancer compound. Mini-Reviews in Medicinal Chemistry. 21(6): 731-737.
Petrocelli, G. and et al. 2023. Plumbagin, a natural compound with several biological effects and anti-inflammatory properties. Life. 13(6): 1303.
Sun, X. and et al. 2020. Piper sarmentosum Roxb.: A review on its botany, traditional uses, phytochemistry, and pharmacological activities. Journal of Ethnopharmacology. 263: 112897.
Hafizah, A.H. and et al. 2010. Piper sarmentosum as an antioxidant on oxidative stress in human umbilical vein endothelial cells induced by hydrogen peroxide. Journal of Zhejiang University-Science B. 11(5): 357-365.
Sireeratawong, S. and et al. 2012. Anti-inflammatory, analgesic, and antipyretic activities of the ethanol extract of Piper interruptum Opiz. and Piper chaba Linn. ISRN Pharmacology. 2012: 480265.
Salleh, W.M. and et al. 2014. Anticholinesterase and antityrosinase activities of ten piper species from malaysia. Advanced Pharmaceutical Bulletin. 4(2): 527-231.
Arulselvan, P. and et al. 2016. Role of antioxidants and natural products in inflammation. Oxidative Medicine and Cellular Longevity. 2016: 5276130.
Roe, K. 2021. An inflammation classification system using cytokine parameters. Scandinavian Journal of Immunology. 93(2): e12970.
Wang, Y. and et al. 2021. Traditional herbal medicine: Therapeutic potential in rheumatoid arthritis. Journal of Ethnopharmacology. 279: 114368.
Wickens, A.P. 2001. Ageing and the free radical theory. Respiration Physiology. 128(3): 379-391.
Foss, K. and et al. 2022. Antioxidant activity and profile of phenolic compounds in selected herbal plant. Plant Foods for Human Nutrition. 77(3): 383-389.
Samuhasaneeto, S. and Yusakul, G. 2021. Modulatory effects of Benjakul extract on rat hepatic cytochrome P450 enzymes. Heliyon. 7(11): e08498.
Turapra, B. and et al. 2016. Determination of active constituents and antioxidative activity in Citrus maxima (Burm.) Merr. Isan Journal of Pharmaceutical Sciences. 11(Suppl.): 80-91. (in Thai)
Dunkhunthod, B. and Sittisart, P. 2022. Antioxidant activity and cytotoxicity against human cancer cell lines of the aqueous leaf extracts of Smilax luzonensis, Hoya kerrii and Derris elliptica. Journal of Science and Technology, Ubon Ratchathani University. 24(2): 1-11. (in Thai)
Ashafa, A.O. and et al. 2010. In vitro antioxidant activity of extracts from the leaves of Felicia muricata thunb. An underutilized medicinal plant in the Eastern Cape Province, South Africa. African Journal of Traditional, Complementary and Alternative Medicines. 7(4): 296-302.
Yee, L.S. and et al. 2023. Optimization of total phenolic content, total flavonoid content and anti-gout properties of polyherbal formulation. Journal of Complementary and Integrative Medicine. 20(4): 772-778.
Skehan, P. and et al. 1990. New colorimetric cytotoxicity assay for anticancer-drug screening. Journal of the National Cancer Institute. 82(13): 1107-1112.
Kalaiselvan, S. and Rasool, M.K. 2016. Triphala herbal extract suppresses inflammatory responses in LPS-stimulated RAW 264.7 macrophages and adjuvant-induced arthritic rats via inhibition of NF-KB pathway. Journal of Immunotoxicology. 13(4): 509-525.
Kahkonen, M.P., Hopia, A.I. and Heinonen, M. 2001. Berry phenolics and their antioxidant activity. Journal of Agricultural and Food Chemistry. 49(8): 4076-4082.
Tripathi, S.K., Panda, M. and Biswal, B.K. 2019. Emerging role of plumbagin: Cytotoxic potential and pharmaceutical relevance towards cancer therapy. Food and Chemical Toxicology. 125: 566-582.
Nobori, T. and et al. 1994. Deletions of the cyclin-dependent kinase-4 inhibitor gene in multiple human cancers. Nature. 368(6473): 753-756.
Dang, G.K. and et al. 2011. Antiinflammatory activity of Phyllanthus emblica, Plumbago zeylanica and Cyperus rotundus in acute models of inflammation. Phytotherapy Research. 25(6): 904-908.
