A Survey of Airborne Fungi and Bacteria in Microbiology Laboratories, Faculty of Science, Ubon Ratchathani University

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Sasithorn Lorroengsil
Nipaporn Khamhlom
Chanyakarn Kokaphan
Yuparat Kruawongsa
Patoomthip Polyon
Siriwaranya Srisakhamkullawat
Kallaya Harnpicharnchai

Abstract

This research was a survey study with the objective to survey the types of airborne fungi and bacteria in 5 microbiology laboratories, Faculty of Science, Ubon Ratchathani University, and compare the concentrations of microorganisms before and after classes. The concentrations of fungi and bacteria were measured using a Single Stage Impactor following the NIOSH Manual of Analytical Methods 0800: Bioaerosols Sampling. The identification of fungi and bacteria was performed by analyzing the nucleotide sequences of fungal ITS regions and bacterial 16S rDNA regions, respectively. The results revealed that airborne microbial concentrations of all laboratories did not exceed the monitoring value according to the Notification of the Department of Health regarding Indoor Air Quality Monitoring Standards in Public Building B.E. 2565. The results of the survey and identification of airborne fungi and bacteria in all microbiology laboratories demonstrated that the most predominant fungi were those of the genus Cladosporium, including Cladosporium tenuissimum, Cladosporium sp. and Cladosporium colombiae, as well as Aspergillus sp. and Trichoderma sp. while the most predominant bacteria were Bacillus megaterium, Paenibacillus illinoisensis and Curtobacterium luteum.

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