Phytochemical Fingerprint Analysis and Antioxidant Activities of Water and Methanolic Extracts of Chrysanthemum morifolium Ramat. using TLC and FTIR-ATR
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Abstract
This research aimed to investigate phytochemical contents, antioxidant activities, major chemical characteristics and preliminary structural features of extracts from flowers of white chrysanthemum (Chrysanthemum morifolium) and yellow chrysanthemum (Chrysanthemum indicum). From the extraction of white and yellow chrysanthemum flowers using water and methanol as extraction solvents, four crude extracts were obtained including methanolic extract of yellow chrysanthemum flower (YM-1), methanolic extract of white chrysanthemum flower (WM-2), aqueous extract of yellow chrysanthemum flower (YD-3) and aqueous extract of white chrysanthemum flower (WD-4). Total phenolic and flavonoid contents of the extracts were analyzed using the Folin-Ciocalteu assay and aluminum chloride colorimetric assay, respectively. The antioxidant activities of the extract were analyzed using DPPH radical scavenging assay, nitric oxide scavenging assay and ferrous ion chelating assay. In addition, major chemical characteristics and preliminary structural features were analyzed using thin-layer chromatography (TLC) and Fourier transform infrared spectroscopy with attenuated total reflectance (FTIR-ATR). The results revealed that the aqueous extracts contained significantly higher levels of phenolic and flavonoid compounds than the methanolic extracts. Among all samples, YD-3 exhibited the highest content of bioactive compounds and the strongest DPPH radical scavenging activity, whereas YM-1 demonstrated the greatest ferrous ion chelating ability. These findings were consistent with FTIR-ATR analysis, which revealed prominent phenolic functional groups associated with antioxidant activity in the aqueous extracts. TLC analysis revealed distinct chemical fingerprints in the methanolic extracts. In conclusion, aqueous extracts of both white and yellow chrysanthemum flowers possess strong potential as natural antioxidant sources. The results of this study provide fundamental scientific information for the future development of chrysanthemum flower-based products.
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บทความที่ได้รับการตีพิมพ์เป็นลิขสิทธิ์ของ วารสารวิทยาศาสตร์และเทคโนโลยี มหาวิทยาลัยอุบลราชธานี
ข้อความที่ปรากฏในบทความแต่ละเรื่องในวารสารวิชาการเล่มนี้เป็นความคิดเห็นส่วนตัวของผู้เขียนแต่ละท่านไม่เกี่ยวข้องกับมหาวิทยาลัยอุบลราชธานี และคณาจารย์ท่านอื่นๆในมหาวิทยาลัยฯ แต่อย่างใด ความรับผิดชอบองค์ประกอบทั้งหมดของบทความแต่ละเรื่องเป็นของผู้เขียนแต่ละท่าน หากมีความผิดพลาดใดๆ ผู้เขียนแต่ละท่านจะรับผิดชอบบทความของตนเองแต่ผู้เดียว
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