Production of Low-Alcohol Beverages from Pummelo Juice (Citrus maxima (Burm.) Merr.) Cultivated Variety of Khao Taeng Kwa, Fermented with the Mycelia of Pink Oyster Mushroom (Pleurotus flabellatus (Berk & Br.) Sacc.)
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Abstract
Low-alcohol beverage was produced from the juice of pummelo (Citrus maxima (Burm.) Merr.), Khao Taeng Kwa cultivated variety, fermented with pink oyster mushroom (Pleurotus flabellatus) mycelia, as a potential alternative alcohol product. The study aimed to determine the optimal pummelo juice concentration, amount of mycelial starter, and the soluble solid content in the pummelo juice for low-alcohol beverage production. Results demonstrated that P. flabellatus mycelia effectively fermented 100% pummelo juice. The optimal fermentation conditions included 15 mycelial starter pieces, each 0.7 cm in diameter, and a soluble solids content of 22 °Brix. After 12–21 days of fermentation, the final product contained 1.00–1.04% alcohol, highlighting its potential as a promising low-alcohol alternative.
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References
Aschariyhaphotha, W. (2004). Development of wine product by mushroom fermentation [Master’s thesis, King Mongkut’s Institute of Technology Ladkrabang]. King Mongkut’s Institute of Technology Ladkrabang Repository. https://opac.lib.kmitl.ac.th/catalog/BibItem.aspx?BibID=b00167740 (in Thai)
Chaijumrus, S. (1993). Partial purification and characterization of antitumor activities of the polysaccharides isolated from fruiting body and the mycelium of Ganoderma lucidum [Master’s thesis, Chulalongkorn University]. Chulalongkorn University Repository. https://cuir.car.chula.ac.th/handle/123456789/62771 (in Thai)
Corfe, S., Hyde, R., & Sheperd, J. (2022). Alcohol-free and low-strength drinks: Understanding their role in reducing alcohol-related harms. Social Market Foundation. https://coilink.org/20.500.12592/0pmdhg
Department of Agricultural Extension. (2007). Thailand's international trade statistics. Information Development and Services Group, Bangkok. (in Thai)
Klaus, A., Wan-Mohtar, W. A. A. Q. I., Nikolić, B., Cvetković, S., & Vunduk, J. (2021). Pink oyster mushroom Pleurotus flabellatus mycelium produced by an airlift bioreactor: The evidence of potent in vitro biological activities. World Journal of Microbiology and Biotechnology, 37(1), 17-30. https://doi.org/10.1007/s11274-020-02980-6
Malathi, G., & Murugesan, A. K. (2023). Bioconversion, nutritional analysis, radical scavenging and characterization of substrates of Pleurotus flabellatus (Berk. and Br.) Sacc. South African Journal of Botany, 157, 423-437. https://doi.org/10.1016/j.sajb.2023.04.025
Ministry of Public Health. (2010). Nutritional value in fruits. Agricultural Co-operative Federation of Thailand, Bangkok. (in Thai)
Norrarach, O. (2007). Study on wine production by mushroom mycelium fermentation [Master’s thesis, Mahasarakham University]. Mahasarakham University Repository. https://opac.msu.ac.th/bibitem?bibid=b00231392 (in Thai)
Office of Provincial Commercial Affairs Chainat. (2007). Report on application for registration of geographical indications. Department of Intellectual Property, Chai Nat Province. (in Thai)
Office of the Forest Herbarium. (2014). Tem Smitinand’s Thai plant names. Department of National Parks, Wildlife and Plant Conservation, Bangkok. (in Thai)
Okamura, T., Ogata, T., Minamoto, N., Takeno, T., Noda, H., Fukuda, S., & Ohsugi, M. (2001). Characteristics of wine produced by mushroom fermentation. Bioscience, Biotechnology, and Biochemistry, 65(7), 1596-1600. https://doi.org/10.1271/bbb.65.1596
Poomkaedum, P. (2003). Studies on genetic variation of Tricholoma crassum isolates by isozyme techniques [Master’s thesis, King Mongkut’s Institute of Technology Ladkrabang]. King Mongkut’s Institute of Technology Ladkrabang Repository. https://opac.lib.kmitl.ac.th/catalog/BibItem.aspx?BibID=b00164948 (in Thai)
Rahman, N. S. A., Hassan, M., Hamid, N. I., Mohamad, S. A., Mohamad, N., Mahadi, M., Zailan, N. F. Z., & Rozila, I. (2021). Antioxidative and photoprotective effects of Pleurotus flabellatus (pink oyster mushroom) polysaccharides against UVA radiation-induced cytotoxicity in human dermal fibroblast (HS-27) cell line. Malaysian Journal of Medicine and Health Sciences, 17(3), 185-191.
Somogyi, M. (1952). Notes on sugar determination. Journal of Biological Chemistry, 195(3), 19-23. https://doi.org/10.1016/S0021-9258(19)50870-5
Sornprasert, R., & Pangnoi, S. (2015). The imitation wine from the juice of pomelo (Citrus maxima (Burm.f.) Merr.), Khao Taeng Kwa variety, fermented with the mycelium of Hed Krang (Schizophyllum commune Fr.). Journal of King Mongkut’s University of Technology North Bangkok, 25(3), 449-457. http://dx.doi.org/10.14416/j.kmutnb.2015.06.001 (in Thai)
Thai Industrial Standards Institute. (2001). Wine industry product standard (TISI. 2089-2001). Ministry of Industry. (in Thai)
Thai Industrial Standards Institute. (2024a). Thai community product standard: Fruit wine (TCPS.2/2024). Ministry of Industry. (in Thai)
Thai Industrial Standards Institute. (2024b). Thai community product standard: Herbal wine (TCPS.31/2024). Ministry of Industry. (in Thai)
Theppakorn, T., & Chaiwong, S. (2011). Bioactive compounds in pummelo and grapefruit. King Mongkut’s Agricultural Journal, 29(2), 90-99. (in Thai)
World Health Organization. (2023). A public health perspective on zero- and low alcohol beverages. World Health Organization. https://www.who.int/publications/i/item/9789240072152