การใช้พีซีอาร์ในการทำดีเอ็นเอมาตรฐานต้นทุนต่ำ
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DNA ladder has been widely used to determine the size of DNA fragments by electrophoresis in routine molecular biology laboratories. Commercial DNA ladders are typically used for comparing the size and estimating the DNA concentration of unknown DNA sample. However, it is quite expensive. Here, we developed a simple strategy for the preparation of 100 bp DNA Ladder which comprises the 12 designed primers to amplify 100 bp to 1,000 bp and 1,500 bp DNA fragments from the vector (pRGEB32) as a template for polymerase chain reaction (PCR). All primers could be used for amplification of all individual fragments in the same PCR profile. Therefore, the total running time of PCR was reduced. In addition, there were no undesired PCR products and the requirement of the purification step. Our procedure for production of the DNA ladder was simple and time saving. The total cost was also inexpensive compared with those of the commercial ones. This result indicates that it can be used for molecular studies.
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บทความที่ได้รับการตีพิมพ์เป็นลิขสิทธิ์ของ PBRU Science Journal
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