Cost PCR Based DNA Ladder
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Abstract
DNA ladder has been widely used to determine the size of DNA fragments by electrophoresis in routine molecular biology laboratories. However, the commercial DNA ladders are quite expensive although they are able to measure the concentration but it is not always necessary in some experiments. Here, we developed a simple strategy for the preparation of 100 bp DNA Ladder which comprises 12 designing primers to amplify 100 bp to 1,000 bp and 1,500 bp DNA fragments from discoverable vector as a template for polymerase chain reaction. Holistic primers design resulted in amplification of fragments in only one PCR program in shorter cycle without any exceeding products and purification step. Our procedure for production of DNA ladder could be simple, time saving, and inexpensive in comparison with commercial ones, indicating that it can be used for molecular studies.
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