Survey of Dioscorea spp. and Effect of Crude Starch Extract on Growth of Bacteria
Article Sidebar
Main Article Content
Abstract
Agroecosystems consist of several interactions of components between plants, microbes and soil in natural environments for maintaining soil fertility. Dioscorea spp. are important tuber plants and serve as a source of carbohydrates containing starch and sugar. Thus, the research aimed to survey tuber plants in Dioscorea spp. from dry forest and discover bacterial community associated with Dioscorea spp. growing in areas without the application of fertilizer. Dioscorea spp. surveyed from dry dipterocarp forest showed highly expressed similarity of physical characteristics to Dioscorea myriantha Kunth and Dioscorea alata L. A total of 56 bacterial isolates divided into the groups of free-living and endophytic bacteria as 28 and 28 isolates were obtained from rhizosphere soil and plant tuber, respectively. All bacteria were preliminarily classified into 31 gram-positive and 25 gram-negative bacterial isolates with the shape of cells as coccus, bacillus, coccobacilli, and filamentous. The most colony characteristics growing on culture medium demonstrated small to large size, white to yellow, circular to irregular shape, flat to convex elevation, and entire to irregular margin. Analysis of total starch content from Dioscorea myriantha Kunth sample exhibited higher content (5.94 g/µL) than Dioscorea alata L. Hydrolyzed starch extract from Dioscorea spp. with acid displayed a higher amount of reducing sugar than non-hydrolyze. The application of crude starch extract supplementing in a culture medium showed highly enhanced growth of the EN1 and FR1 isolates. Therefore, discovered Dioscorea spp., from the forest were found bacterial population living in the plant and rhizosphere soil by exhibiting the different morphologies. In addition, the plants comprised the average total starch without different values. This starch could be digested to the monosaccharide and applied as the carbon source of culture media to support the energy for microbial growth.
Article Details
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
บทความที่ได้รับการตีพิมพ์เป็นลิขสิทธิ์ของ PBRU Science Journal
References
อาทิตยา พองพรหม. ความรู้เบื้องต้นด้านนิเวศเกษตรเพื่อการประยุกต์ใช้ในระบบเกษตรกรรมยังยืน. สำพัฒนาและถ่ายทอดเทคโนโลยีสำนักงานการปฏิรูปที่ดินเพื่อเกษตรกรรม 2561;588:1-62.
Wilkin P, Thapyai C. Dioscoreaceae: Flora of Thailand. Forest Herbarium, Royal Forest Department 2009;10:1-40.
Wickramasinghe HAM, Takigawa S, Matsuura-Endo C, Yamauchi H, Noda T. Comparative analysis of starch properties of different root and tuber crops of Sri Lanka. Food Chem 2009;112:98-103. https://doi.org/10.1016/j.foodchem.2008.05.046.
Wanasundera JPD, Ravindran G. Nutritional assessment of yam (Dioscorea alata) tubers. Plant Foods Hum Nutr 1994;46:33-39. https://doi.org/10.1007/BF01088459.
Srivichai S, Hongsprabhas P. Profiling anthocyanins in Thai purple Yams (Dioscorea alata L.). Int J Food Sci 2020;2020:1-10. https://doi.org/10.1155/2020/1594291.
Kihara S, Yamamoto K, Hisatomi A, Shiwa Y, Chu CC, Takada K, et al. Bacterial community of water Yam (Dioscorea alata L.) cv. A-19. Microbes Environ 2022;37:1-8. https://doi.org/10.1264/jsme2.ME21062.
Subroto E, Jeanette G, Meiyanasari Y, Luwinsky I, Baraddiaz S. Review on the analysis methods of starch, amylose, amylopectinin food and agricultural products. Int J Emerg Trends Eng Dev 2020;8:3519-24. https://doi.org/10.30534/ijeter/2020/103872020.
Bahdanovich P, Axelrod K, Khlystov AY, Samburova V. Optimized spectrophotometry method for starch quantification. Analytica 2022;3:394-405. https://doi.org/10.3390/analytica3040027.
Negrulescu A, Patrulea V, Mincea MM, Ionascu C, Vlad-Oros BM, Ostafe V. Adapting the reducing sugars method with dinitrosalicylic acid to microtiter plates and microwave heating. J Braz Chem Soc 2012;23:2176-82. https://doi.org/10.1590/S0103-50532013005000003.
น้องนุช สารภี, ปิยรัตน์ มีแก้ว, ยุพเยาว์ โตคีรี, ชัยพันธุ์ สารภี. ความหลากชนิดของพืชหัวกินได้ในท้องถิ่นจังหวัดสุรินทร์. PSRU J Sci Tech 2019;4:43-53. https://li01.tci-thaijo.org/index.php/atj/article/view/251441.
Shajeela PS, Mohan VR, Louis Jesudas L, Tresina Soris P. Nutrition and antnutrition evaluation of wild yam (Dioscorea spp.). Tropical and Subtropical Agroecosystems, 2011;14:723-30.
พิรดา สุุดประเสุริฐ, กรณัฏฐ์ นาคภิบาล, วิจิตร์ วิโสรัมย์. ลักษณะเม็ดแป้งและโครงสร้างทางเคมีของแป้งจากมันท้องถิ่นกลุ่ม Dioscorea spp. วารสารวิทยาศาสตร์และเทคโนโลยี 2;31:18-32.
Lee BH, Eskandari R, Jones K, Reddy KR, Quezada-Calvillo R, Nichols BL, et al. Modulation of starch digestion for slow glucose release through “Toggling” of activities of mucosal β-glucosidases. THE J Biol Chem 2012;287:31929-38. https://doi.org/10.1074/jbc.M112.351858.
Petushkova E, Mayorova E, Tsygankov A. TCA cycle replenishing pathways in photosynthetic purple non-sulfur bacteria growing with acetate. Life 2021;11:1-37. https://doi.org/10.3390/life11070711.
Far BE, Ahmadi Y, Khosroushahi AY, Dilmaghani A. Microbial alpha-amylase production: progress, challenges and perspectives. Adv Pharm Bull 2020;10:350-8. https://doi.org/10.34172/apb.2020.043.
Michlmayr H, Schümann C, Wurbs P, Nuno M, Silva BB, Rogl V, et al. A β -glucosidase from Oenococcus oeni ATCC BAA-1163 with potential for aroma release in wine: cloning and expression in E. coli. World J Microbiol Biotechnol 2010;26:1281-9. https://doi.org/10.1007/s11274-009-0299-5.
