Development of skin care product from leaves of Moringa oleifera herbal extracts with antioxidant activities
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Abstract
Herbal extracts are widely used to cure the unhealthy and damaged skins by pollution and free radical caused sunlight. Studies have shown revealed many herbs with good possess antioxidant properties. Therefore, natural extracts are increasingly being used as active ingredients in cosmeceutical products. The research aimed to 1) investigate the antioxidant activities of ethanol extracts from 5 herbal plants which consist of leaves of Moringa oleifera, Morus alba, Centella asiatica, flower of Clitoria ternate, and root of Daucus carota 2) develop a cream product from herbal extracts that have antioxidant effect and 3) study physical and chemical stability of product. The research approach is broken down into the following 3 steps: The first step is preparation of herbal extracts by maceration method. In the second step, antioxidant activities were studied using
2,2-diphenyl-1-picrylhydrazyl radical (DPPH), 2,2’-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) and Ferric reducing ability power (FRAP) assay. The third step involves developing a cream product and testing for physical and chemical stability under accelerated temperature conditions. The results showed that leaves of Moringa oleifera extract possessed the highest antioxidant activity in DPPH (304.254 ± 26.665 µg/ml) and FRAP (2.031 ± 0.255 mM Fe2+/ mg) methods. While ABTS method showed the highest antioxidant effect of root of Daucus carota extract with a VEAC value of 0.172 ± 0.040 mM/mg. Therefore, leaves of Moringa oleifera extract was chosen to be developed as a cream for skin care compared with control cream under accelerated temperature conditions. From the results of the stability study, we have found that the cream formula from leaves of Moringa oleifera extract has stable DPPH antioxidant value (78.619 - 95.853%). The texture of the cream was not separated into layers, but its color and scent were changed slightly. While the pH value (6.12 to 6.30) and viscosity value (10,900.00 to 14,626.66 cP) were found to comply with the standards for herbal skin care products.
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