Alpha-Glucosidase Inhibitory Activity, Antioxidant Activity, and Phytochemical Content of Launaea sarmentosa root.
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Abstract
Diabetes is a growing global health concern. Inhibition of α-glucosidase is a key mechanism for controlling blood glucose levels. The study of the antidiabetic and antioxidant activities of Launaea sarmentosa root is therefore a promising approach for the development of safe natural medicines. This study aimed to investigate the α-glucosidase inhibitory activity, antioxidant activity, and phytochemical content of the ethanol extract from Launaea sarmentosa roots. Root samples were collected from Bang Khrok Subdistrict, Ban Laem District, Phetchaburi Province. The extract was prepared using ethanol maceration and tested for α-glucosidase inhibition using a colorimetric assay, antioxidant activity using the DPPH assay, and phytochemical quantification, including total phenolics (Folin-Ciocalteu), total flavonoids (AlCl₃), and total alkaloids (Bromocresol Green).
Results showed IC₅₀ values of 0.35 and 0.34 mg/ml for maltase and sucrase inhibition, respectively. The extract exhibited 64.97 ± 0.34% antioxidant activity. Phytochemical analysis revealed total phenolic content of 13.512 ± 0.214 mg GAE/g extract, total flavonoid content of 238.231 ± 2.019 mg QE/g extract, and total alkaloid content of 0.411 ± 0.039 mg AE/g extract. The findings of this study can serve as preliminary data for identifying the active compounds in the roots of L.sarmentosa for potential applications in pharmaceuticals and dietary supplements.
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References
International Diabetes Federation. Diabetes facts and figures [Internet]. 2021 [cited 2025 Jan 28]. Available from: https://idf.org
World Health Organization. Global report on diabetes. Geneva: World Health Organization; 2022.
International Diabetes Federation. Diabetes facts and figures show the growing global burden for individuals, families, and countries. IDF Diabetes Atlas [Internet]. 2021 [cited 2025 Jan 28]. Available from: https://idf.org/about-diabetes/diabetes-facts-figures/
Thailand faces diabetes crisis as new cases per year cross 300,000 mark [Internet]. 2025 [cited 2025 Jan 28]. Available from: https://www.nationthailand.com/thailand/general/40032838
Ota A, Ulrih NP. α-Glucosidase inhibitors from the natural sources and their beneficial effects on human health. Curr Top Med Chem 2017;17:411-31.
Tundis R, Loizzo MR, Menichini F. Natural products as α-glucosidase and α-amylase inhibitors and their hypoglycemic potential in the treatment of diabetes: An update. Mini Rev Med Chem 2010;10:315-31.
Sriplang K, Adisakwattana S, Rungsipipat A, Yibchok-Anun S. Effects of Morus alba on plasma glucose and lipid profiles in normal and streptozotocin-induced diabetic rats. Phytother Res 2007;21:396-400.
Kawamura-Konishi Y, Watanabe N, Saito M, Nakajima N, Sakaki T, Katayama T. Food-derived polyphenols as potential α-glucosidase inhibitors. J Agric Food Chem 2014;62:8932-8.
Kooti W, Farokhipour M, Asadzadeh Z, Ashtary-Larky D, Asadi-Samani M. The role of medicinal plants in the treatment of diabetes: A systematic review. Electron Physician 2016;8:1832-42.
Xu Y, Gu Y, Zhang C. Advances on the bioactivity, chemical composition, and utilization of α-glucosidase inhibitors. Food Chem 2021;347:128930.
Basile A, Santoro R, Cione E. Phytochemical composition and bioactivity of medicinal plants: A review. Nat Prod Res 2018;32:1824-34.
Khanna K, Thakur M, Sharma M. Phytochemical analysis and medicinal properties of some Indian medicinal plants. Int J Pharmacogn Phytochem Res 2020;12:137-44.
Le Thi Them L, Dung PTN, Trinh PTN, Hung QT, Vi LNT, Tuan NT, et al. Saponin, polyphenol, flavonoid content and α-glucosidase inhibitory activity, antioxidant potential of Launaea sarmentosa leaves grown in Ben Tre province, Vietnam. IOP Conf Ser Mater Sci Eng 2019;542:012036.
Sannigrahi S, Mazumder UK, Pal D, Mishra ML, Maity S. Flavonoids of Enhydra fluctuans exhibit analgesic and anti-inflammatory activity in different animal models. Pak J Pharm Sci 2011;24:369-75.
Salih Y, Harisha CR, Shukla VJ, Acharya R. Pharmacognostical evaluation of Launaea sarmentosa (Willd.) Schultz Bip. ex Kuntze root. AYU 2013;34:90-4.
Das S, Priyanka KR, Prabhu K, Vinayagam R, Rajaram R, Kang SG. Anticandidal properties of Launaea sarmentosa among the salt marsh plants collected from Palk Bay and the Gulf of Mannar coast, Southeastern India. Antibiotics 2024;13:748.
Moghal MMR, Millat MS, Hussain MS, Islam MR. Thrombolytic and membrane stabilizing activities of Launaea sarmentosa. Int J Pharmacogn 2016;3:354-8.
Nguyen TQ, Hong TT, Khang VT, Pham DT, Giao DH, Tran TTT, et al. Anti-inflammatory constituents isolated from Launaea sarmentosa against infection by LPS-stimulated macrophages. Rec Nat Prod 2024;18:663-73.
Gunde MC, Wani JS, Pethe AM. Pharmacognostic and phytochemical studies on leaves of Launaea sarmentosa (Willd.) Schultz-Bip. Ex Kuntze. Int J Ayurvedic Med 2022;13:759–63.
Them LE, Dung PTN, Trinh PTN, Hung QT, Vi LNT, Tuan NT. Saponin, polyphenol, flavonoid content and α-glucosidase inhibitory activity, antioxidant potential of Launaea sarmentosa leaves grown in Ben Tre province, Vietnam. IOP Conf Ser. 2019.
Ramadhan R, Phuwapraisirisan P. New arylalkanones from Horsfieldia macrobotrys, effective antidiabetic agents concomitantly inhibiting α-glucosidase and free radicals. Bioorg Med Chem Lett 2015;25:4529-33.
Karagozler AA, Erdag B, Emek YC, Uygun DA. Antioxidant activity and proline content of leaf extracts from Dorystoechashastata. Food Chem 2008;111:400-7.
Singleton VL, Orthofer R, Lamuela-Raventós RM. Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Methods Enzymol 1999;299:152–78.
Zhishen J, Mengcheng T, Jianming W. Determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chem 1999;6:555-9.
Fazel S, Hamidreza M, Rouhollah G, Mohammadreza V. Spectrophotometric determination of total alkaloids in some Iranian medicinal plants. J Appl Hortic 2010;12:69-70.
Zhang B, Deng Z, Ramdath DD, Tang Y, Chen PX, Liu R, et al. Phenolic profiles of ten oat varieties and their antioxidant and α-glucosidase inhibitory activities. Food Chem 2020;315:126227.
Kim GN, Jang HD, Kim CI. Antioxidant capacity of Morus alba leaf extracts and their inhibitory effects on α-glucosidase. J Med Plants Res 2011;5:1652-8.
Chen W, Su H, Xu Y, Bao T, Zheng X. Protective effect of Glycyrrhiza glabra extract against oxidative stress-induced hepatotoxicity in rats. J Funct Foods 2016;27:500-7.
Mohana T, Athira K, Anu A. Antioxidant potential of Curcuma longa and its protective role against oxidative stress induced diseases. Asian Pac J Trop Biomed 2012;2:703-11.
Shahidi F, Ambigaipalan P. Phenolics and polyphenolics in foods, beverages and spices: Antioxidant activity and health effects-A review. J Funct Foods 2015;18:820-97.
Heinrich M, Mah J, Amirkia V. Alkaloids used as medicines: Structural phytochemistry meets biodiversity-An update and forward look. Molecules 2021;26:1836.
