Alkaloid and Phenolic Content and Antioxidant Activity of Some Bitter Herbs
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Abstract
This study aimed to (1) analyze the alkaloid content, phenolic content, and antioxidant activity in selected bitter medicinal plants, and (2) investigate the relationship between phenolic and alkaloid contents and the antioxidant activity of these plants. The selected bitter medicinal plants included Senna siamea, Andrographis paniculata, Morinda citrifolia, and Azadirachta indica, which were collected from Tha Saen Subdistrict, Ban Lat District, Phetchaburi Province. The leaf parts of the plant were extracted using the maceration method with ethanol.The extracts were analyzed for alkaloid content using the Bromocresol Green method, phenolic content using the Folin-Ciocalteu method, and antioxidant activity using the DPPH assay. The statistics used included mean, standard deviation, statistical analysis using one-way analysis of variance (ANOVA) at a confidence level of p≤0.05, and the correlation coefficient (R).
The results showed that the alkaloid content in the bitter plant extracts ranged from 1.333 to 6.494 mg AE/g crude extract, with Andrographis paniculata containing the highest alkaloid content (6.494 ± 0.187 mg AE/g crude extract). The phenolic content ranged from 242.61 to 794.89 µg GAE/g crude extract, with Senna siamea having the highest phenolic content (794.89 ± 15.03 µg GAE/g crude extract). At a concentration of 1 mg/mL, the antioxidant activity of the bitter plant extracts ranged between 24.05 - 58.17%, with Andrographis paniculata exhibiting the highest antioxidant activity (58.17%). Furthermore, a strong positive correlation (r = 0.6394) was observed between alkaloid content and antioxidant activity. In contrast, phenolic content showed a weak negative correlation (r = -0.1456) with antioxidant activity.
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บทความที่ได้รับการตีพิมพ์เป็นลิขสิทธิ์ของ PBRU Science Journal
References
Mishra A, Sharma A, Patil R. Bitter plant extracts as a source of bioactive compounds: A review. J Med Plants Res 2020;14:45–59.
Nguyen TT, Tran QH, Le HP. Phenolic compounds and alkaloids as major contributors to the bitterness and antioxidant activity of medicinal plants. Phytochemistry 2022;198:113123.
Zhang L, Wu F, Yang Y. Plant alkaloids: Biosynthesis, bioactivities, and future perspectives. Front Plant Sci 2021;12:680226.
Wink M. Modes of action of herbal medicines and plant secondary metabolites. Med Res Rev 2020;40:1291–351.
Facchini PJ. Alkaloid biosynthesis in plants: Biochemistry, cell biology, molecular regulation, and metabolic engineering applications. Annu Rev Plant Physiol Plant Mol Biol 2001;52:29–66.
Dai J, Mumper RJ. Plant phenolics: Extraction, analysis and their antioxidant and anticancer properties. Molecules 2010;15:7313–52.
Rice-Evans CA, Miller NJ, Paganga G. Antioxidant properties of phenolic compounds. Trends Plant Sci 1997;2:152–9.
Panche AN, Diwan AD, Chandra SR. Flavonoids: An overview. J Nutr Sci 2016;5:e47.
Cheynier V, Comte G, Davies KM, Lattanzio V, Martens S. Plant phenolics: Recent advances on their biosynthesis, genetics, and ecophysiology. Plant Physiol Biochem 2013;72:1–20.
Pham-Huy LA, He H, Pham-Huy C. Free radicals, antioxidants in disease and health. Int J Biomed Sci 2008;4:89–96.
Lobo V, Patil A, Phatak A, Chandra N. Free radicals, antioxidants and functional foods: Impact on human health. Pharmacogn Rev 2010;4:118–26.
Shahidi F, Ambigaipalan P. Phenolics and polyphenolics in foods, beverages and spices: Antioxidant activity and health effects–A review. J Funct Foods 2015;18:820–97.
Kumar A, Sharma S, Pandey S. Bitter plant alkaloids and their pharmacological significance: A review. J Herb Med 2020;30:100473.
Singh R, Verma P, Pandey S. Evaluation of antioxidant properties in selected bitter herbs. J Herb Med 2021;27:100420.
Doughari JH. Phytochemicals: Extraction methods, basic structures, and mode of action as potential chemotherapeutic agents. In: Phytochemicals - A Global Perspective of Their Role in Nutrition and Health 2012;1:1–32.
Syah YM, Aimi N, Kitajima M, Achmad SA, Takayama H. Cassiarin A and B, novel antimalarial alkaloids from Cassia siamea. Tetrahedron Lett 2001;42:6941–3.
Okokon JE, Nwafor PA, Ekpo AJ. Anti-inflammatory and analgesic activities of leaf extract of Senna siamea. Pak J Pharm Sci 2010;23:290–5.
Mishra SK, Sangwan NS, Sangwan, RS. Andrographis paniculata (Kalmegh): A Review. Phcog Rev 2007;1:283-98.
Akbar S. Andrographis paniculata: A review of pharmacological activities and clinical effects. Altern Med Rev 2011;16:66–77.
Palu AK, Kim AH, West BJ, Deng S, Jensen J, White L. The effects of Morinda citrifolia L. (noni) on the immune system: Its molecular mechanisms of action. J Ethnopharmacol 2009;123:423–8.
Wang MY, Su C, Peng L. Morindine: A new alkaloid from Morinda citrifolia. J Nat Prod 2001;64:1130–1.
Deng S, West BJ, Jensen CJ. A quantitative comparison of phytochemical components in global noni fruits and their commercial products. Food Chem 2010;122:267–70.
Subapriya R, Nagini S. Medicinal properties of neem leaves: A review. Curr Med Chem Anticancer Agents 2005;5:149–56.
Kumar V, Basu N, Bhattacharyya D. Neem (Azadirachta indica): Nature’s Drugstore. Asian Pac J Trop Biomed 2013;3:505–14.
Fazel S, Hamidreza M, Rouhollah G, Mohammadreza V. Spectrophotometric determination of total alkaloids in some Iranian medicinal plants. J Appl Hortic 2010;12:69–70.
Singleton VL, Orthofer R, Lamuela-Raventós RM. Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Methods Enzymol 1999;299:152–78.
Karagozler AA, Erdag B, Emek YC, Uygun DA. Antioxidant activity and proline content of leaf extracts from Dorystoechas hastata. Food Chem 2008;111:400–7.
Chao W, Lin B. Isolation and identification of bioactive compounds in Andrographis paniculata (Chuanxinlian). Chinese Medicine 2010;5:17 [Internet]. [cited 2025 March 4]. Available from: http://www.cmjournal.org/content/5/1/17
Li H, Deng Z, Liu R, Young JC, Zhu H, Loewen S, Tsao R. Characterization of phytochemicals and antioxidant activities of a yellow onion-derived polyphenolic-rich extract. Food Chem 2019;276:295–303.
Chaisrisook C, Pithayanukul P, Bavovada R, Leanpolchareanchai J, Saparpakorn P. Antioxidant and hepatoprotective activities of Senna siamea leaves extract. J Ethnopharmacol 2016;194:618–25.
รัตนา วงศ์ชูพันธ์, พิณพิศา เทพราช, กมลชนก สุริยมาตร, เพ็ญนภา ชูยงค์, แน่งน้อย แสงเสน่ห์. ผลของตัวทำละลายต่างชนิดที่ใช้ในการสกัดลำต้นโคลงเคลงต่อฤทธิ์ต้านอนุมูลอิสระและสารพฤกษเคมี. PBRU Science J 2567;21:30-44.
Gupta R, Sharma M, Kumar A. Phenolic compounds and their role in antioxidant activity: A review. J Med Plants Res 2020;14:45–60.
Kumar P, Patel M, Rao S. Alkaloids as natural antioxidants and their potential applications. Food Chem 2021;342:128–36.
Zhang Y, Xu H, Wang Y, Dong J, Zhao X. Phenolic composition and antioxidant activity of different tissues of Senna siamea. J Food Biochem 2022;46:e13987.
