Characterization and Properties of Chitosan/PVA Bio-based Film Incorporated with Clitoria ternatea L. (Butterfly pea) Extract and Its Application in Foods


  • Putkrong Phanumong Division of Food Safety Management and Technology, Faculty of Science and Technology, Rajamangala University of Technology Krungthep


Butterfly pea, Anthocyanin, pH-sensing, Film properties


Natural anthocyanin pigments gained awareness to use to improve the function of food packaging based on dye sensitivities when chemical composition changes. In this study Clitoria ternatea L. (Butterfly pea) extract (10-30%) was incorporated into chitosan/poly-vinyl alcohol (PVA) based film to produce pHsensing elements. Physical, mechanical and barrier properties of film, e.g., swelling index, thickness, water vapor permeability coefficient (WVPC) and tensile strength were evaluated. Film incorporated with 30% Clitoria ternatea L. extract showed an increase in film thickness and decreased in tensile strength and swelling index. No significant difference (p>0.05) was observed in WVPC between treatments. Films were immersed in six levels of pH buffer (1, 3, 5, 7, 9 and 12) and exhibited color response ranging from brownish-red (acidic), bluish-green (neutral) and yellow (basic), respectively, within 15 min which was confirmed by color parameters (L*, a*, b*, chroma and hue angle). Total color different (ΔE) greatly rose under extreme pH conditions (pH 1 and 12). Films were also performed on a model of low (pasteurized milk and chicken meat) and high (fresh-cut pineapple) acid foods. The visual color of film responded when pH shifted, which changed into pale pink (pasteurized milk), green (chicken meat) and red (fresh-cut pineapple) compared to an initial color (bluish-green). This study revealed that an embedding of Clitoria ternatea L. extract into chitosan/PVA based films had a pH-sensing potential material for application in smart packaging.


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Abraham, A., Soloman, P.A., & Rejini, V.O. (2016). Preparation of chitosan-polyvinyl alcohol blends and studies on thermal and mechanical properties. Procedia Technology, 24, 741-748.

Ahmad, A.N., Lim, S.A., & Navaranjan, N. (2020). Development of sago (Metroxylon sagu)-based colorimetric indicator incorporated with butterfly pea (Clitoria ternatea) anthocyanin for intelligent food packaging. Journal of Food Safety, 40(4), e12807.

Antoniolli, L.R., Benedetti, B.C., Filho, S., Moreira, M.S., Garruti, D. dos S., & Borges, M. de F. (2012). Shelf life of minimally processed pineapples treated with ascorbic and citric acids. Bragantia, 71(3), 447-453.

ASTM. (1989). Annual book of ASTM standards. Philadelphia, PA: American Society for testing and Materials.

Caner, C., Vergano, P.J., & Wiles, J.L. (1998.) Chitosan film mechanical and permeation properties as affected by acid, plasticizer, and storage. Journal of Food Science, 63(6), 1049-1053.

Cavalcanti, O.A., den Mooter, G., Caramico-Soares, I., & Kinget, R. (2002.) Polysaccharides as excipients for colon-specific coatings, permeability and swelling properties of casted films. Drug Development and Industrial Pharmacy, 28(2), 157-164.

Chen, X., & Gu, Z. (2013). Absorption-type optical pH sensitive film based on immobilized purple cabbage pigment. Sensors and Actuators B: Chemical, 178, 207-211.

Choi, I., Lee, J.Y., Lacroix, M., & Han, J.(2017). Intelligent pH indicator film composed of agar/potato starch and anthocyanin extracts from purple sweet potato. Food Chemistry, 218, 122–128.

Golasz, L.B., Silva, J.D., & Silva, S.B.D. (2013). Film with anthocyanins as an indicator of chilled pork deterioration. Food Science and Technology, 33, 155-162.

Hidayat, S.H., Dirpan, A., Adiansyah, Djalal, M., Rahman, A.N.F., & Ainani, A.F. (2019). Sensitivity determination of indicator paper as smart packaging elements in monitoring meat freshness in cold temperature. IOP Conference Series: Earth and Environmental Science, 343, 012076.

Hidayati, N.A., Wijaya, M.W., Bintoro, V.P., Mulyani, S., & Pratama, Y. (2021). Development of biodegradable smart packaging from chitosan, polyvinyl alcohol (PVA) and butterfly pea flower’s (Clitoria ternatea L.) anthocyanin extract. Food Research, 5(3), 307-314.

Jayasekara, R., Harding, I., Bowater, I., Christie, G.B.Y., & Lonergan, G.T. (2004). Preparation, surface modification and characterisation of solution cast starch PVA blended films. Polymer Testing Journal, 23, 17-27.

Kungsuwan, K., Singh, K., Phetkao, S., & Utama-ang, N. (2014). Effects of pH and anthocyanin concentrationon color and antioxidant activity of Clitoria ternatea extract. Food and Applied Bioscience Journal, 2(1), 31-46.

Kuswandi, B., Maryska, C., Abdullah, A., & Heng, L.Y. (2013.) Real time on-package freshness indicator for guavas packaging. Journal of Food Measurement and Characterization, 7(1), 29-39.

Liu, B., Xu, H., Zhao, H., Liu, W., Zhao, L., & Li, Y. (2017). Preparation and characterization of intelligent starch/ PVA films for simultaneous colorimetric indication and antimicrobial activity for food packaging applications. Carbohydrate Polymers, 157, 842-849.

Liu, Y., Wang, S., & Lan, W. (2018). Fabrication of antibacterial chitosan-PVA blended film using electrospray technique for food packaging applications. International Journal of Biological Macromolecules, 107, 848–854.

Liu, Y., Wang, S., Lan, W., & Qin, W. (2019). Development of ultrasound treated polyvinyl alcohol/tea polyphenol composite films and their physicochemical properties. Ultrasonics Sonochemistry, 51, 381-394.

Lu, Y., Li, L., Yang, F. X., & Zhang, H. G. (2013a). Application of chitosan composite film to sliced fresh Channa argus for shelf life extension. Advanced Materials Research, 781-784, 1550–1557.

Lu, L., Zheng, W., Lv, Z., & Tang, Y. (2013b). Development and application of time-temperature indicators used on food during the cold chain logistics. Packaging Technology and Science, 26, 80-90.

Mangaraj, S., Yadav, A., Bal, L.M., Dash, S.K., & Mahanti, N.K. (2019). Application of biodegradable polymers in food packaging industry: A comprehensive review. Journal of Packaging Technology and Research, 3(1), 77-96.

Miranda, S.P., Garnica, O., Lara-Sagahon, V., & Cárdenas, G. (2004). Water vapor permeability and mechanical properties of chitosan composite films. Journal of the Chilean Chemical Society, 49(2), 173-178.

Nwabor, O.F., Sudarshan, S., Paosen, S., Vongkamjan, K., & Voravuthikunchai, S.P. (2020). Enhancing food shelf life with polyvinyl alcohol-chitosan polymer nanocomposite films from bioactive Eucalyptus leaf extracts. Food Bioscience, 100609.

Park, S.Y., Marsh, K.S., & Rhim, J.W. (2002). Characteristics of different molecular weight chitosan films affected by the type of organic solvents. Journal of Food Science, 67(1), 194-197.

Pereira, J.R., Arruda, I.N., & Stefani, R. (2015). Active chitosan/ PVA films with anthocyanins from Brassica oleraceae (Red Cabbage) as Time Temperature Indicators for application in intelligent food packaging. Food Hydrocolloids, 43, 180-188.

Pham, T.N., Nguyen, D.C., Lam, T.D., Thinh, P.V., Le, X.T., Nguyen, D.V.V., ... Bach, L.G. (2019). Extraction of anthocyanins from Butterfly pea (Clitoria ternatea L. Flowers) in Southern Vietnam: Response surface modeling for optimization of the operation conditions. Materials Science and Engineering, 542, 012032.

Prietto, L., Mirapalhete, T.C., Pinto, V.Z., Hoffmann, J.F., Vanier, N.L., Lim, L.T., ... Zavareze, E.R. (2017). pH-sensitive films containing anthocyanins extracted from black bean seed coat and red cabbage. LWT– Food Science and Technology, 80, 492–500.

Ranganna, S. (1986). Handbook of analysis and quality control for fruit and vegetable products. New Delhi: Tata McGraw-Hill. Schaefer, D., & Cheung, W.M. (2018). Smart packaging: Opportunities and challenges. Procedia CIRP, 72, 1022-1027.

Shukla, V. G., Kandeepan, M., Vishnuraj, R., & Soni. A. (2016). Anthocyanins based indicator sensor for intelligent packaging application. Agricultural Research, 5(2), 205–209.

Silva-Pereira, M.C., Teixira, J.A., & Pereira-Junior, V.A. (2015). Chitosan/corn starch blend films with extract from Brassica oleraceae (red cabbage) as a visual indicator of fish deterioration. LWT – Food Science and Technology, 61, 258-262.

Sinha, K., Das (Saha), P., & Datta, S. (2012). Natural blue dye from Clitoria Ternatea: Extraction and analysis methods. Research Journal of Textile and Apparel, 16(2), 34–38.

Siripatrawan, U., & Harte, B.R. (2010). Physical properties and antioxidant activity of an active film from chitosan incorporated with green tea extract. Food Hydrocolloids, 24, 770-775.

Sukprasong, P. (2013). Study on color stability of Butterfly pea extract and preparation of poly(vinyl alcohol) films entrapped with Butterfly pea. (Master thesis). Bangkok: King Mongkut University of Technology Thonburi, Bangkok.

Tripathi, S., Mehrotra, G.K., & Dutta, P.K. (2009). Physicochemical and bioactivity of cross-linked chitosan–PVA film for food packaging applications. International Journal of Biological Macromolecules, 45, 372–376.

Turan, D. (2021). Water vapor transport properties of polyurethane films for packaging of respiring foods. Food Engineering Reviews, 13, 54–65.

Vanderroost, M., Ragaert, P., Devlieghere, F., & Meulenaer, B.D. (2014). Intelligent food packaging: The next generation. Trends in Food Science & Technology, 39(1), 47-62.

Vu, C., & Won, K. (2013). Novel water-resistant UV-activated oxygen indicator for intelligent food packaging. Food Chemistry, 140, 52-56.

Wu, D., Hou, S. Chen, J., Sun, Y., Ye, X., & Liu, D. (2015). Development and characterization of an enzymatic time-temperature indicator (TTI) based on Aspergillus niger lipase. LWT - Food Science and Technology, 60, 1100-1104.

Yang, W., Fortunati, E., Bertoglio, F., Owczarek, J.S., Bruni, G., Kozanecki, M., ... Puglia, D. (2018). Polyvinyl alcohol/chitosan hydrogels with enhanced antioxidant and antibacterial properties induced by lignin nanoparticles. Carbohydrate Polymers, 181, 275-284.

Yousefi, H., Ali, M.M., Su, H.M., Filipe, C.D.M., & Didar, T.F. (2018). Sentinel wraps: Real-time monitoring of food contamination by printing dNAzyme probes on food packaging. ACS Nano, 12(4), 3287-3294.

Zivanovic, S., Chi, S., & Draughon, A.F. (2005). Antimicrobial activity of chitosan films enriched with essential oils. Journal of Food Science, 70(1), M45–M51.

Zhang, B.-Y., Samapundo, S., Pothakos, V., de Baenst, I., Sürengil, G., Noseda, B., & Devlieghere, F. (2013). Effect of atmospheres combining high oxygen and carbon dioxide levels on microbial spoilage and sensory quality of fresh-cut pineapple. Postharvest Biology and Technology, 86, 73-84.

Zhang, X., Lu, S., & Chen, X. (2014). A visual pH sensing film using natural dyes from Bauhinia blakeana Dunn. Sensor and Actuators B, 198, 268-273.

Zhang, H., Wu, J., & Guo, X. (2016). Effects of antimicrobial and antioxidant activities of spice extracts on raw chicken meat quality. Food Science and Human Wellness, 5(1), 39–48.

Zhang, L., Liu, Z., Wang, X., Dong, S., Sun, Y., & Zhao, Z. (2019). The properties of chitosan/zein blend film and effect of film on quality of mushroom (Agaricus bisporus). Postharvest Biology and Technology, 155, 47-5.




How to Cite

Phanumong, P. (2023). Characterization and Properties of Chitosan/PVA Bio-based Film Incorporated with Clitoria ternatea L. (Butterfly pea) Extract and Its Application in Foods. Journal of Food Health and Bioenvironmental Science, 14(3), 1–11. Retrieved from



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