Degradation of Poly (lactide), Poly (butylene succinate) and Poly (butylene Succinate/poly (lactide) by UV Irradiation in Combination with Enzymatic Hydrolysis Method

Authors

  • Thanasak Lomthong Faculty of Science and Technology, Rajamangala University of Technology Thanyaburi, Pathum Thani, 12110 Thailand
  • Pradabrat Prajanket Faculty of Science and Technology, Rajamangala University of Technology Thanyaburi, Pathum Thani, 12110 Thailand
  • Jednipit Borthong Faculty of Science and Technology, Rajamangala University of Technology Thanyaburi, Pathum Thani, 12110 Thailand
  • Praphasiri Akarach Faculty of Science and Technology, Rajamangala University of Technology Thanyaburi, Pathum Thani, 12110 Thailand
  • Kewalin Kunok Faculty of Science and Technology, Rajamangala University of Technology Thanyaburi, Pathum Thani, 12110 Thailand
  • Waranya Loilert Faculty of Science and Technology, Rajamangala University of Technology Thanyaburi, Pathum Thani, 12110 Thailand
  • Sudarat Naksiri Faculty of Science and Technology, Rajamangala University of Technology Thanyaburi, Pathum Thani, 12110 Thailand
  • Panuwat Prasertsri Faculty of Science and Technology, Rajamangala University of Technology Thanyaburi, Pathum Thani, 12110 Thailand
  • Kannika Kromsaeng Faculty of Science and Technology, Rajamangala University of Technology Thanyaburi, Pathum Thani, 12110 Thailand

Keywords:

UV-degradation, Bioplastic degradation, Enzymatic hydrolysis, Lipase, Alkaline protease

Abstract

This study elucidated an approach for hydrolysis of bioplastics by UV irradiation in combination with enzymatic hydrolysis. The hydrolytic enzymes, including commercial lipase and alkaline protease, were used for hydrolysis bioplastics of poly (lactide) (PLA), poly (butylene succinate) (PBS), and poly (butylene succinate)/poly (lactide) blend (PBS/PLA) at pH 9.0, 50°C for 72 h. The results showed that each enzyme could hydrolyze all kinds of bioplastics. The combination of commercial enzymes improved the degradation of PLA, PBS, and PBS/PLA blend, which showed the highest weight loss of bioplastic concentration (100 g/L) yielded, 37.70±1.23, 32.60±1.15 and 34.87±3.44%, respectively. The optimum temperature and pH of the hydrolysis reaction were found at 50°C and 9.5, respectively, which gave the highest percent degradation at 39.13±0.71, 35.77±1.94 and 37.90±1.99%, respectively when using each bioplastic at 100 g/L. The exposure of UV irradiation at 254 nm for 36 and subsequent hydrolysis with mixed enzymes at pH 9.5, 50°C for 36 h increased the percent degradation up to 48.45±2.85, 42.8±2.56, and 44.1±1.75%, respectively. The hydrolysis of PLA, PBS, and PBS/PLA blend in a 2.0 L stirrer fermenter led to a percent degradation at 49.50±2.29, 44.33±1.52, and 48.17±3.01%, respectively. Scanning electron microscope (SEM) confirmed the change of the physical structures of the degradation products. These results showed the alternative approach to reduce the bioplastic wastes by applying the UV irradiation with hydrolytic enzymes that could develop at an industrial level in the future.

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Published

2023-09-26

How to Cite

Lomthong, T., Prajanket, P., Borthong, J., Akarach, P., Kunok, K., Loilert, W., Naksiri, S., Prasertsri, P., & Kromsaeng, K. (2023). Degradation of Poly (lactide), Poly (butylene succinate) and Poly (butylene Succinate/poly (lactide) by UV Irradiation in Combination with Enzymatic Hydrolysis Method. Journal of Food Health and Bioenvironmental Science, 14(3), 12–19. Retrieved from https://li01.tci-thaijo.org/index.php/sdust/article/view/260599

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