Synergistic and Counter Effect of Biocides, Amines and Emulsifier in the Combinatorial Toxicity Study
Keywords:Combinatorial Toxicity, Biocide, Biosensor, Amine
Pollution is one of the major environmental issues that affect human beings. Metalworking fluids are widely used in many industries. There are many chemical components such as amines and biocides in the metalworking fluids, which cannot be biologically treated, and disposal is still a problem. Often chemicals are tested for toxicity individually, however, there are interactions between combinations of chemicals. Hence, in this research, chemicals that are commonly used in metalworking fluids, are tested in combination as part of a factorial experimental design. Three types of commercially available biocides (A14, AEF, AOX - coded due to commercial rights), two amines (Monoethanolamide - MEA, Triethylolamine - TEA), and an emulsifier (blinded because of commercial rights) were tested. A bacterial biosensor E.coli HB101 was used to assess toxicity. A total of 63 tests were carried out. It was found that the toxic responses do not align with predictions based on the sum of the responses to individual compounds. Instead, there are interactions that cause synergistic or counter effects. For example, biocides A14 and AEF were found to be lethally toxic; biocide AOX and MEA were found to be slightly toxic. The combination of MEA, AEF, A14 was found to be the most toxic of the 63 possible combinations. However, when AOX was added, the toxicity level decreased in indication that toxicity was mitigated. This study shows that understanding the combinatorial toxicity could help to inform eco-design and promote sustainable biological treatment at the end of product life.
Azimi, Y., & Thompson, P.I. (2017). Phosphorus depletion as a green alternative to biocides for controlling biodegradation of metalworking fluids. Environmental Science and Technology, 51(10), 5695-5702.
Bardgett, R., Usher, M., & Hopkins, D. (2005). New perspectives in soil biodiversity. In D. Wall, (Ed.), Biological Diversity and Function in Soils (pp.1-10). Cambridge: Cambridge University Press.
BASF Agricultural Solutions-Global Website. (2022). Mixture oxicity [online]. Retrieved January 16, 2022 from https://agriculture.basf.com/global/en/media/positions-on-agriculture/mixture-toxicity.html
Belden, J.B., Gilliom, R.J., & Lydy, M.J. (2007). How well can we predict the toxicity of pesticide mixtures to aquatic life? Integrated Environmental Assessment and Management, 3(3), e1–e5.
BP-Castrol Limited. (2012). Formulation of Syntilo 9913 metalworking fluid. Personal communication. 10th January 2012.
Byers, J.P. (1994). Metalworking fluids. New York: Marcel Dekker Inc. Byers, J.P. (2006). Metalworking fluids. Boca Raton, FL: CRC/ Taylor & Francis.
Canadian Centre for Occupational Health & Safety. (2005). Metalworking fluids. CCOHs [online]. Retrieved January, 29, 2020 from http://www.ccohs.ca/oshanswers/chemicals/metalworking_fluids.htchanml
Chazal, P. (1995). Pollution of modern metalworking fluids containing biocides by pathogenic bacteria in France. European Journal of Epidemiology, 11(1), 1-7.
Cheng, C., Phippsa, D., & Alkhaddarb, R.M. (2005). Treatment of spent metalworking fluids. Water Research, 39(17), 4051-4063.
Chipasa, K. (2011). Best practice guide for the disposal of water-mix metalworking fluids. United Kingdom Lubricants Association (UKLA) [online]. Retrieved January, 6, 2022 from http://www.ukla.org.uk/documents/Best%20Practice%20Guide_Final.pdf
Cole, L., Bradford, M.A., Shaw, P.J.A., & Bardgett, R.D. (2006). The abundance, richness and functional role of soil meso and macrofauna in temperate grassland - A case study. Applied Soil Ecology, 33(2), 186-198.
Gauthier, S.L. (2003). Metalworking fluids: Oil mist and beyond. Applied Occupational and Environmental Hygiene, 18(11), 818-824.
Glenn, T.F., & van Antwerpen, F. (1998). Opportunities and market trends in metalworking fluids. Journal of the Society of Tribologists and Lubrication Engineers, 54, 31-37.
Health and Safety Executive. (2015). Biocides: The basics. Health and Safety Executive [online]. Retrieved February, 6, 2022 from http://www.hse.gov.uk/biocides/basics.htm
Hussain, A., Audira, G., Malhotra, N., Uapipatanakul, B., Chen, J.R., Lai, Y.H., ... Hsiao, C.D. (2020). Multiple screening of pesticides toxicity in zebrafish and daphnia based on locomotor activity alterations. Biomolecules, 10(9), 1224.
Jiang, Y., Marang, L., Tamis, J., van Loosdrecht, M.C., Dijkman, H., & Kleerebezem, R. (2012). Waste to resource: Converting paper mill wastewater to bioplastic. Water Research, 46(17), 5517-5530.
Jones, I.A., & Joshi, L.T. (2021). Biocide use in the antimicrobial era: A review. Molecules, 26(8), 2276.
Kelly, J.R., & Harwell, M.A. (1989). Indicators of ecosystem response and recovery. In S. Levin, M. Harwell, J. Kelly, and K. Kimball, (Eds.), Ecotoxicology: Problems and approaches (pp. 9-35). New York: Springer-Verlag.
Koeman, J.H. (1998). Pollution and its ecotoxicological consequences. In J. Lynch and A.Wiseman, (Eds.), Environmental biomonitoring: The biotechnology ecotoxicology interface (pp.17-26). New York: Cambridge University Press.
Koskella, J. & Stotzky, G. (1997). Microbial utilization of free and clay-bound insecticidal toxins from bacillus thuringiensis and their retention of insecticidal activity after incubation with microbes. Applied and Environmental Microbiology, 63(9), 3561-3568.
Kreyszig, E., (2011). Advanced engineering mathematics (10th ed.). Hoboken, N.J.: John Wiley & Sons.
Liess, M., Henz, S., & Shahid, N. (2020). Modeling the synergistic effects of toxicant mixtures. Environmental Sciences Europe, 32(1), 1-10.
Luostarinen, S., Luste, S., & Sillanpää, M. (2009). Increased biogas production at wastewater treatment plants through co-digestion of sewage sludge with grease trap sludge from a meat processing plant. Bioresource Technology, 100(1), 79-85.
Madigan, M.T., Martinko, J.M., & Parker, J. (2000). Microorganisms and microbiology. In Brock Biology of Microorganisms (9th ed.) (pp.1-24). London: Prentice Hall International.
Malhotra, N., Chen, K.H.C., Huang, J.C., Lai, H.T., Uapipatanakul, B., Roldan, M. J.M., ... Hsiao, C.D. (2021). Physiological effects of neonicotinoid insecticides on non-target aquatic animals-An updated review. International Journal of Molecular Sciences, 22(17), 9591.
Massei, R., Busch, W., Wolschke, H., Schinkel, L., Bitsch, M., Schulze, T., Krauss, M., & Brack, W. (2018). Screening of pesticide and biocide patterns as risk drivers in sediments of major European River Mouths: Ubiquitous or River Basin-Specific contamination? Environmental Science and Technology, 52(4), 2251–2260.
McCoy, J.S. (1994). Introduction: Tracing the historical development of metalworking fluids. In J.P. Byers, (Ed.), Metalworking fluids (2nd ed.) (pp.1–18). Boca Raton, FL: Taylor & Francis Group.
Mwinyikione, M. (2011). Essentials of ecotoxicology in the tanning industry. Journal of Environmental Chemistry and Ecotoxicology, 3(13), 323-331.
Northwest Aerospace Alliance. (2013). Blaser Swisslube’s New Grinding Product Has The ‘G Factor’. Northwest Aerospace Alliance [online]. Retrieved January, 13, 2022 from http://www.aerospace.co.uk/news/130614-jemtech
Procter & Gamble (2005). Ecotoxicity. Science-in-the-box [online] Retrieved March 17, 2022 from http://www.scienceinthebox.com/en_UK/safety/ecotoxicity_en.html
Ralf, B., & Schäfer, J.J.P. (2016). Advancing understanding and prediction in multiple stressor research through a mechanistic basis for null models. International Journal of Laboratory Hematology, 38(1), 42–49.
Reisch, M.S. (2012). A revival for biocides. Chemical and Engineering News, 90(5), 30-31.
Russell, A.D. (2003). Biocide use and antibiotic resistance: The relevance of laboratory findings to clinical and environmental situations. Lancet Infectious Diseases, 3(12), 794–803.
Samuel, J., Rafiee, J., Dhiman, P., Yu, Z., & Koratkar, N. (2011). Graphene Colloidal suspensions as high performance semi-synthetic metal-working fluids. The Journal of Physical Chemistry C, 115(8), 3410-3415.
Singh, N., Gupta, V., Kumar, A., & Sharma, B. (2017). Synergistic effects of heavy metals and pesticides in living systems. Frontiers in Chemistry, 5, 70. Strachan, G., Preston, S., Maciel, H., Porter, A.J.R., & Paton, G.I. (2001). Use of bacterial biosensors to interpret the toxicity and mixture toxicity of herbicides in freshwater. Water Research, 35(14), 3490–3495.
Tchobanoglous, G., Burton, F.L., & Stensel, H.D. (2004). Wastewater engineering: Treatment and reuse (4th ed.). Boston: McGraw-Hill.
Thompson, I.P., & van der Gast, C.J. (2010). The Microbiology of metalworking fluids. In Handbook of Hydrocarbon and Lipid Microbiology (pp. 2369-2376). Berlin: Springer-Berlin Heidelberg.
Tiensing, T., Strachan, N., & Paton, G.I. (2002). Evaluation of interactive toxicity of chlorophenols in water and soil using lux-marked biosensors. Journal of Environmental Monitoring, 4(4), 482-489.
Truhaut, R., (1977). Ecotoxicology: Objectives, principles and perspectives. Ecotoxicology and Environmental Safety, 1(2), 151–173.
Uapipatanakul, B. (2015). Harmonising metal working fluid formulations with end-of-life biological treatment (Doctoral dissertation). University of Oxford, Department of Engineering Science, Environmental Engineering.
Uapipatanakul, B. (2020). Green technology, [Unpublished manuscript]. Chemistry Department, Faculty of Science and Technology, Rajamangala University of Technology Thanyaburi.
Walker, C.H., Hopkin, S.P., Sibly, R.M., & Peakall, D.B. (1996). Principles of ecotoxicology. London: Taylor Francis.
Walker, M.J., Montemagno, C.D., & Jenkins, M.B. (1998). Source water assessment and nonpoint sources of acutely toxic contaminants: A review of research related to survival and transport of Cryptosporidium parvum. Water Resources Research, 34(12), 3383–3392.
White, D.C., Flemming, C.A., Leung, K.T., & Macnaughton, S.J. (1998). In situ microbial ecology for quantitative appraisal, monitoring, and risk assessment of pollution remediation in soils, the subsurface, the rhizosphere and in biofilms. Journal of Microbiological Methods, 32(2), 93-105.
Willing, A. (2001). Lubricants based on renewable resources – An environmentally compatible alternative to mineral oil products. Chemosphere, 43(1), 89-98.
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