Influence of Exogenous Sucrose on Total Phenolic, Vitamin C and Antioxidant Enzymes of Soybean (Glycine max L.) Sprouts
Keywords:
Soybean sprouts, Sucrose, SOD, CAT, APXAbstract
This research explained the effect of exogenous sucrose on the levels of DPPH free radical scavenging ability, total phenolics and vitamin C as well as the L-galactono-1,4-lactone dehydrogenase (GalLDH), superoxide dismutase (SOD), catalase (CAT) and ascorbate peroxidase (APX) activities of soybean (Glycine max L.) sprouts. Soybean seeds were soaked in the various solutions prepared with 0, 1, 10 and 100 mM sucrose at 25°C for 12 hr and then sprayed with these solutions every 12 hr during the germination for 5 days. The higher concentration of exogenous sucrose decreased the L* value of soybean sprouts; whereas, the levels of DPPH free radical scavenging ability, total phenolic, vitamin C and GalLDH, SOD, CAT and APX activities increased. The soybean sprouts from 100 mM sucrose-treated seeds showed the maximum levels of DPPH free radical scavenging ability, total phenolics, vitamin C and GalLDH, SOD, CAT and APX activities, i.e. 85%, 164.95 mg GAE g-1 FW, 120.65 mg 100g-1 FW, 25.32 U g-1FW, 12.00 U g-1FW, 25.36 U g-1FW and 924 U g-1FW, respectively. Thus, it suggested that the sucrose treatment helped to promote the soybean sprouts in containing high-levels of antioxidant activities.
References
Aghdam, M.S., Luo, Z., Li, L., Jannatizadeh, A., Fard, J.R., & Pirzad, F. (2020). Melatonin treatment maintains nutraceutical properties of pomegranate fruits during cold storage. Food Chemistry, 303, 125385.
Aghdam, M.S., Mukherjee, S., Flores, F.B., Arnao, M.B., Luo, Z., & Corpas, F.J. (2022). Functions of melatonin during postharvest of horticultural crops. Plant and Cell Physiology, 63(12), 1764-1786.
Alscher, R.G., Donahue, J.L., & Cramer, C.L. (1997). Reactive oxygen species and antioxidants: relationships in green cells. Physiologia Plantarum, 100, 224-233.
Bridges, S.M., & Salin, M.L. (1981). Distribution of ironcontaining superoxide dismutase in vascular plants. Plant Physiology, 68, 275-278.
Cao, Y., Yang, M., Chen, S., Zhou, Z., Li, X., Wang, X., & Bai, J. (2015). Exogenous sucrose influences antioxidant enzyme activities and reduces lipid peroxidation in water-stressed cucumber leaves. Biological Plantarum, 59, 147-153.
Cao, Y., Yang, M., Li, X., Zhou, Z., Wang, X., & Bai, J. (2014). Exogenous sucrose increases chilling tolerance in cucumber seedlings by modulating antioxidant enzyme activity and regulating proline and soluble sugar contents. Scientia Horticulturae, 179, 67-77.
Chen, L., Song, Y., Li, S., Zhang, L., Zou, C., & Yu, D. (2012). The role of WRKY transcription factors in plant abiotic stresses. BBA Gene Regulatory Mechanisms, 1819, 120-128.
Chen, L., Wu, J. E., Li, Z., Liu, Q., Zhao, X., & Yang, H. (2019). Metabolomic analysis of energy regulated germination and sprouting of organic mung bean (Vigna radiata) using NMR spectroscopy. Food Chemistry, 286, 87-97.
Couée, I., Sulmon, C., Gouesbet, G., & Amrani, A. (2006). Involvement of soluble sugars in reactive oxygen species balance and responses to oxidative stress in plants. Journal of Experimental Botany, 57, 449-459.
Dumont, S., & Rivoal, J. (2019). Consequences of oxidative stress on plant glycolytic and respiratory metabolism. Frontiers in Plant Science, 10, 166.
Eastmond, P.J. (2006). SUGAR-DEPENDENT1 Encodes a patatin domain triacylglycerol lipase that initiates storage oil breakdown in germinating arabidopsis seeds. The Plant Cell, 18, 665-675.
Ebert, A.W., Chang, C., Yan M., & Yang, R. (2017). Nutritional composition of mung bean and soybean sprouts compared to their adult growth stage. Food Chemistry, 237, 15-22.
Eltayeb, A.E., Kawano, N., Badawi, G.H., Kaminaka, H., Sanekata, T., Shibahara, T., ... Tanaka, K. (2007). Overexpression of monodehydroascorbate reductase in transgenic Tobacco confers enhanced tolerance to ozone, salt and polyethylene glycol stresses. Planta, 225, 1255-1264.
Giannopolitis, C.N., & Ries, S.K. (1977). Superoxide dismutase. I. occurrence in higher plants. Plant Physiology, 59, 309-314
Guo, R., Yuan, G., & Wang, Q. (2011). Effect of sucrose and mannitol on the accumulation of health-promoting compounds and the activity of metabolic enzymes in broccoli sprouts. Scientia Horticulturae, 128, 159-165.
Guo, X., Li, T., Tang, K., & Liu, R. (2012). Effect of germination on phytochemical profiles and antioxidant activity of mung bean sprouts (Vigna radiata). Journal of Agricultural and Food Chemistry, 60, 11050-11055.
He, Y., Liu, Y., Chen, Q., Bian, A., & Chen. W. (2001). Comparison of the optimal pH for 4 antioxidant enzymes in the seedlings of tall fescue (Festuca arundinacea Schreb) and kentucky bluegrass (Poa pratensis L.). Journal of Nanjing Agricultural University, 24, 1-4.
Kampfenkel, K., Vanmontagu, M., & Inze, D. (1995). Extraction and determination of ascorbate and dehydroascorbate from plant tissue. Analytical Biochemistry, 225, 165-167.
Koodkaew, I. (2019). NaCl and glucose improve healthpromoting properties in mung bean sprouts. Scientia Horticulturae, 247, 235-241.
Kraboun, K. (2019). A combination of fermentation method and germinated brown rice (Oryza sativa) to enhance antioxidant activity of angkak. Journal of Microbiology, Biotechnology and Food Sciences, 8, 1290-1293.
Li, D., Limwachiranon, J., Li, L., Du, R., & Luo, Z. (2016). Involvement of energy metabolism to chilling tolerance induced by hydrogen sulfide in cold-stored banana fruit. Food Chemistry, 208, 272-278.
Li, D., Zhang, X., Li, L., Aghdam, M.S., Wei, X., Liu, J., ... Luo, Z. (2019). Elevated CO2 delayed the chlorophyll degradation and anthocyanin accumulation in postharvest strawberry fruit. Food Chemistry, 285, 163-170.
Liang, N., & Kitts, D.D. (2014). Antioxidant property of coffee components: assessment of methods that define mechanisms of action. Molecules, 19, 19180-19208.
Lobo, V., Patil, L., Pathak, A., & Chandra, N. (2010). Free radicals, antioxidants and functional foods: Impact on human health. Pharmacognosy Review, 8, 118-126.
López-Amorós, M. L., Hernández T., & Estrella, I. (2006). Effect of germination on legume phenolic compounds and their antioxidant activity. Journal of Food Composition and Analysis, 19, 277-283.
McCord, J.M., & Fridovich, I. (1969). Superoxide dismutase. An enzymatic function for erythrocuprein (hemocuprein). Journal of Biological Chemistry, 244, 6049-6055.
Murugkar, D.A. (2014). Effect of sprouting of soybean on the chemical composition and quality of soymilk and tofu. Journal of Food Science and Technology, 51, 915-92.
Nakano, Y., & Assada, K. (1981). Hydrogen peroxide is scavenged by ascorbate specific peroxidase in spinach chloroplasts. Plant and Cell Physiology, 22, 867-880.
Nishikawa, F., Kato, M., Hyodo, H., Ikoma, Y., Sugiura, M., & Yano, M. (2005). Effect of sucrose on ascorbate level and expression of genes involved in the ascorbate biosynthesis and recycling pathway in harvested broccoli florets. Journal of Experimental Botany, 409, 65-72.
Pertiwi, S.F., Aminah S., & Nurhidayah, J. (2013). Antioxidant activity, chemical characteristics and organoleptic properties of black soybean sprouts (Glycine soja) based on germination time variations. Jurnal Pangan dan Gizi, 4, 10-21.
Price, J., Li, T.C., Kang, S.G., Na, J.K., & Jang, J.C. (2003). Mechanisms of glucose signaling during germination of arabidopsis. Plant Physiology, 132, 1-15.
Qiu, Z., Liu, S., Ni, Z., Liu, H., & Shao, X. (2015). Effect of exogenous sugar treatment on Vc Content and antioxidant activity of mung bean sprouts. Science and Technology of Food Industry, 36, 357-360.
Shao, X., Wang, H., Xu, F., & Cheng, S. (2013). Effects and possible mechanisms of tea tree oil vapor treatment on the main disease in postharvest strawberry fruit. Postharvest Biology and Technology, 77, 94-101.
Sharma, A., Shahzad, B., Rehman, A., Bhardwaj, R., Landi, M., & Zheng, B. (2019). Response of phenylpropanoid pathway and the role of polyphenols in plants under abiotic stress. Molecules, 24, 2452.
Smirnoff, N., Conklin, P.L., & Loewus, F.A. (2001). Biosynthesis of ascorbic acid in plants: A renaissance. Annual Review of Plant Physiology and Plant Molecular Biology, 52, 437-467.
Tabata, K., Oba, K., Suzuki, K., & Esaka, M. (2001). Generation and properties of ascorbic acid-deficient transgenic tobacco cells expressing antisense RNA for L-galactono-1, 4-lactone dehydrogenase. The Plant Journal, 27, 139-148.
Tang, D., Dong, Y., Ren, H., Li, L., & He, C. (2014). A review of phytochemistry, metabolite changes and medicinal uses of the common food mung bean and its sprouts (Vigna radiata). Chemistry Central Journal, 8, 1-9.
Wang, H., Gui, M., Tian, X., Xin, X., Wang, T., & Li, J. (2017). Effects of UV-B on vitamin C, phenolics, flavonoids and their related enzyme activities in mung bean sprouts (Vigna radiata). International Journal of Food Science and Technology, 52, 827-833.
Wei, Y., Wang, X., Shao, X., Xu, F., & Wang, H. (2019). Sucrose treatment of mung bean seeds results in increased vitamin C, total phenolics and antioxidant activity in mung bean sprouts. Food Science and Nutrition, 00, 1-8.
Wheeler, G., Jones, M., & Smirnoff, N. (1998). The biosynthetic pathway of vitamin C in higher plants. Nature, 393, 365-369.
Winarsi, H., Septiana, A.T., & Wulandari, S.P. (2020). Germination improves sensory, phenolic, protein content and anti-inflammatory properties of red kidney bean (Phaseolus vulgaris L.) sprouts milk. Food Research, 4, 1921-1928.
Xu, F., Tang, Y., Dong, S., Shao, X., Wang, H., Zheng, Y., & Yang, Z. (2016). Reducing yellowing and enhancing antioxidant capacity of broccoli in storage by sucrose treatment. Postharvest Biology and Technology, 112, 39-45.
Xu,Y., Charles, M.T., Luo, Z., Mimee, B., Tong, Z., Veronneau, P.-Y., Rolland, D., & Roussel, D. (2019). Ultraviolet-C priming of strawberry leaves against subsequent Mycosphaerella fragariae infection involves the action of reactive oxygen species, plant hormones and terpenes. Plant Cell & Environment, 42, 815-831
Xue-Feng, Z., Jun, L.V., Shahid, M., Anjum, S.A., Na-Jia, L., Xiu-Juan, H., ... San-Gen, W. (2019). Biomass accumulation, photosynthetic pigments, osmotic adjustments and antioxidant activities of leymus chinensis in response to BA, BR and GA. Planta Daninha, 37, e019187872.
Yu, J., Lee, H., Heo, H., Jeong, H.S., Sung, J., & Lee, J. (2023). Sucrose-induced abiotic stress improves the phytochemical profiles and bioactivities of mung bean sprouts. Food Chemistry, 400, 134069.
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