In vitro Antioxidant, Anti-Inflammatory, and Wound Healing Properties of Aloe Vera Infused with Canola Oil for Possible Application in Skin Cosmetics

Khwunjit itsarasook; Piyanuch  Prompamorn; Jittarawadee  Tanghiranrat; Amornrat  Srisukong; Orapin  Komutiban; Woraphot  Haritakun; Kanlayaporn  Chantree

Authors

Khwunjit itsarasook Faculty of Science, Ramkhamhaeng University, Bangkok, 10240 Thailand
Piyanuch Prompamorn Faculty of Science and Technology, Suan Dusit University, Bangkok, 10700 Thailand
Jittarawadee Tanghiranrat Faculty of Science and Technology, Suan Dusit University, Bangkok, 10700 Thailand
Amornrat Srisukong Faculty of Science and Technology, Suan Dusit University, Bangkok, 10700 Thailand
Orapin Komutiban Faculty of Science and Technology, Suan Dusit University, Bangkok, 10700 Thailand
Woraphot Haritakun Faculty of Science and Technology, Suan Dusit University, Bangkok, 10700 Thailand
Kanlayaporn Chantree Faculty of Science and Technology, Kanchanaburi Rajabhat University, Kanchanaburi, 71190 Thailand

Keywords:

Aloe vera oil, Antioxidant, Anti-inflammatory, Wound healing, Skin cosmetic

Abstract

This research investigates the antioxidant, anti-inflammatory, and woundhealing properties of Aloe vera oil, along with its cytotoxic effects on normal human dermal fibroblasts (NHDF), particularly in the context of its potential use as a natural antioxidant in skincare formulations. A. vera oil was extracted using an infusion method with canola oil, and its properties were thorough characterized. The concentration of aloin and cycloartenol in the A. vera oil were measured using high-performance liquid chromatography (HPLC). The resulted indicated that A. vera oil exhibited no cytotoxicity towards NHDF cells. Additionally, the oil demonstrated antioxidant activity, as evidenced by its ability to scavenge 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals, and its reducing potential assessed through free radical scavenging activity (FRAP) assays. Regarding its anti-inflammatory properties, the A. vera oil at a specific concentration showed no cytotoxic effects on RAW 264.7 macrophage cells. Notably, its potent anti-inflammatory effects significantly (p<0.05) reduced nitric oxide (NO) productions in RAW 264.7 macrophage cells stimulated by lipopolysaccharide (LPS), compared to the LPS-treated cells group. Moreover, NHDF cells treated with A. vera oil exhibited a statistically significant (p<0.05) improvement in wound closure area in a scratch wound assay, indicating enhanced cell migration. Collectively, these findings highlight the antioxidant, anti-inflammatory, and wound-healing properties of A. vera oil, underscoring its potential as an eco-friendly and beneficial ingredient for cosmetic skincare products.

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