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Prasaplai is a Thai herbal remedy used in obstetrics and gynecology. In this study, Prasaplai microemulsions (MEs) were developed for topical use, using two types of oil: isopropyl myristate (IPM) and tea tree oil, with polysorbate 80 and butanol as the surfactant and co-surfactant, respectively. The stability of Prasaplai MEs under accelerated conditions was studied. Prasaplai MEs from both oil types had a clear yellow color, with a diameter between 236 and 257 nm. The pH values of all ME were between 5.14 and 5.45, and the viscosities were between 19 and 26 centipoises, with Newtonian flow behavior. After storage under accelerated conditions, the tea tree oil-based MEs showed phase separation, whereas the IPM-based MEs were homogeneous. The size of the tea tree oil-based MEs increased, while pH decreased after storage. The decrease in pH indicated the presence of degradation products in the formulations. The IPM-based Prasaplai MEs reduced in size, and the pH value increased. The amount of remaining curcumin was 73.80% in the tea tree oil-based MEs and 92.20 % in the IPM-based MEs. In conclusion, oil types and ingredients in Prasaplai affects the stability of the MEs. IPM-based Prasaplai MEs might be better for use as topically applied remedies.
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