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The impact of varying concentrations of porcine follicular fluid (pFF) derived from three ovarian follicle types and produced during folliculogenesis on porcine oviductal epithelial cell (pOEC) culture was studied for 24 h using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay. Cells treated with pFF at a protein concentration of 500 µg/mL (from small- and large-sized ovarian follicles) and 600 µg/mL (from medium-sized ovarian follicles) showed the highest viability, which was significantly different from that observed in the control group (p<0.05) and not significantly higher than that observed in the positive control group.This study demonstrated that the impact of pFF on pOECs can be used as a model for biotechnological studies. Further, the study showed that instead of costly fetal calf serum, pFF produced during folliculogenesis can be used as a supplement in culture media to promote porcine oviductal epithelium cell growth and development.
Algriany, O., Bevers, M., Schoevers, E., Colenbrander, B., and Dieleman, S. (2004). Follicle size-dependent effects of sow follicular fluid on in vitro cumulus expansion, nuclear maturation and blastocyst formation of sow cumulus oocytes complexes. Theriogenology, 62(8), 1483-1497.
Areekijseree, M., and Chuen-Im, T. (2012). Effects of porcine follicle stimulating hormone, luteinizing hormone and estradiol supplementation in culture medium on ultrastructures of porcine cumulus oocyte complexes (pCOCs). Micron, 43(2-3), 251-257.
Areekijseree, M., and Veerapraditsin, T. (2008). Characterization of porcine oviductal epithelial cells, cumulus cells and granulosa cells-conditioned media and their ability to induce acrosome reaction on frozen-thawed bovine spermatozoa. Micron, 39(2), 160-167.
Areekijseree, M., and Vejaratpimol, R. (2006). In vivo and in vitro study of porcine oviductal epithelial cells, cumulus oocyte complexes and granulosa cells: A scanning electron microscopy and inverted microscopy study. Micron, 37(8), 707-716.
Bianchi, F., Careri, M., Mangia, A., Musci, M., Santini, S. E., and Basini, G. (2007). Porcine follicular fluids: Comparison of solid-phase extraction and matrix solid-phase dispersion for the GC-MS determination of hormones during follicular growth. Journal of Pharmaceutical and Biomedical Analysis, 44(3), 711-717.
Boncler, M., Różalski, M., Krajewska, U., Podsędek, A., and Watala, C. (2014). Comparison of PrestoBlue and MTT assays of cellular viability in the assessment of anti-proliferative effects of plant extracts on human endothelial cells. Journal of Pharmacological and Toxicological Methods, 69(1), 9-16.
Brachova, P., Alvarez, N. S., Van Voorhis, B. J., and Christenson, L. K. (2017). Cytidine deaminase Apobec3a induction in fallopian epithelium after exposure to follicular fluid. Gynecologic Oncology, 145(3), 577-583.
Chang, S. C. S., Jones, J. D., Ellefson, R. D., and Ryan, R. J. (1976). The porcine ovarian follicle: I. selected chemical analysis of follicular fluid at different developmental stages. Biology of Reproduction, 15(3), 321-328.
Chen, S., Einspanier, R., and Schoen, J. (2013). Long-term culture of primary porcine oviduct epithelial cells: Validation of a comprehensive in vitro model for reproductive science. Theriogenology, 80(8), 862-869.
Ducolomb, Y., González-Márquez, H., Fierro, R., Jiménez, I., Casas, E., Flores, D., Bonilla, E., Salazar, Z., and Betancourt, M. (2013). Effect of porcine follicular fluid proteins and peptides on oocyte maturation and their subsequent effect on in vitro fertilization. Theriogenology, 79(6), 896-904.
Funahashi, H., and Day, B. N. (1993). Effects of follicular fluid at fertilization in vitro on sperm penetration in pig oocytes. Journal of Reproduction and Fertility, 99(1), 97-103.
Gosden, R. G., Hunter, R. H. F., Telfer, E., Torrance, C., and Brown, N. (1988). Physiological factors underlying the formation of ovarian follicular fluid. Journal of Reproduction and Fertility, 82(2), 813-825.
Ito, M., Iwata, H., Kitagawa, M., Kon, Y., Kuwayama, T., and Monji, Y. (2008). Effect of follicular fluid collected from various diameter follicles on the progression of nuclear maturation and developmental competence of pig oocytes. Animal Reproduction Science, 106(3-4), 421-430.
Kim, N. H., Funahashi, H., Abeydeera, L. R., Moon, S. J., Prather, R. S., and Day, B. N. (1996). Effects of oviductal fluid on sperm penetration and cortical granule exocytosis during fertilization of pig oocytes in vitro. Journal of Reproduction and Fertility, 107(1), 79-86.
Kor, N. M. (2014). The effect of corpus luteum on hormonal composition of follicular fluid from different sized follicles and their relationship to serum concentrations in dairy cows. Asian Pacific Journal of Tropical Medicine, 7, S282-S288.
Miessen, K., Sharbati, S., Einspanier, R., and Schoen, J. (2011). Modelling the porcine oviduct epithelium: a polarized in vitro system suitable for long-term cultivation. Theriogenology, 76(5), 900-910.
Oberlender, G., Murgas, L. D. S., Zangeronimo, M. G., da Silva, A. C., de Alcantara Menezes, T., Pontelo, T. P., and Vieira, L. A. (2013). Role of insulin-like growth factor-I and follicular fluid from ovarian follicles with different diameters on porcine oocyte maturation and fertilization in vitro. Theriogenology, 80(4), 319-327.
Orisaka, M., Tajima, K., Tsang, B. K., and Kotsuji, F. (2009). Oocyte-granulosa-theca cell interactions during preantral follicular development. Journal of Ovarian Research, 2(1), 9.
Pongsawat, W., and Youngsabanant, M. (2019). Porcine cumulus oocyte complexes (pCOCs) as biological model for determination on in vitro cytotoxic of cadmium and copper assessment. Songklanakarin Journal of Science and Technology, 41(5), 1029-1036.
Revelli, A., Delle Piane, L., Casano, S., Molinari, E., Massobrio, M., and Rinaudo, P. (2009). Follicular fluid content and oocyte quality: from single biochemical markers to metabolomics. Reproductive Biology and Endocrinology, 7(1), 40.
Sanmanee, N., and Areekijseree, M. (2009). In vitro toxicology assessment of cadmium bioavailability on primary porcine oviductal epithelial cells. Environmental Toxicology and Pharmacology, 27(1), 84-89.
Sanmanee, N., and Areekijseree, M. (2010). The effects of fulvic acid on copper bioavailability to porcine oviductal epithelial cells. Biological Trace Element Research, 135(1-3), 162-173.
Somigliana, E., Vigano, P., La Sala, G. B., Balasini, M., Candiani, M., Incerti, L., Busacca, M., and Vignali, M. (2001). Follicular fluid as a favourable environment for endometrial and endometriotic cell growth in vitro. Human Reproduction, 16(6), 1076-1080.
Spitzer, D., Murach, K. F., Lottspeich, F., Staudach, A., and Illmensee, K. (1996). Different protein patterns derived from follicular fluid of mature and immature human follicles. Human Reproduction, 11(4), 798-807.
Tsafriri, A., Pomerantz, S. H., and Channing, C. P. (1976). Inhibition of oocyte maturation by porcine follicular fluid: partial characterization of the inhibitor. Biology of Reproduction, 14(5), 511-516.
Van de Wiel, D., Bar-Ami, S., Tsafriri, A., and De Jong, F. (1983). Oocyte maturation inhibitor, inhibin and steroid concentrations in porcine follicular fluid at various stages of the oestrous cycle. Journal of Reproduction and Fertility, 68(1), 247-252.
Van Meerloo, J., Kaspers, G. J., and Cloos, J. (2011). Cell sensitivity assays: the MTT assay. Cancer Cell Culture: Methods and Protocols, 731, 237-245.
Vatzias, G., and Hagen, D. R. (1999). Effects of porcine follicular fluid and oviduct-conditioned media on maturation and fertilization of porcine oocytes in vitro. Biology of Reproduction, 60(1), 42-48.
Way, A. L. (2006). Isolation and culture of bovine oviductal epithelial cells for use in the anatomy and physiology laboratory and undergraduate research. Advances in Physiology Education, 30(4), 237-241.
Youngsabanant-Areekijseree, M., Tungkasen, H., Srinark, C. and Chuen-Im, T. (2019). Determination of porcine oocyte and follicular fluid proteins from small, medium, and large follicles for cell biotechnology research. Songklanakarin Journal of Science and Technology, 41(1), 192-198.
Youngsabanant, M., and Mettasart, W. (2020). Changes in secretory protein of porcine ampulla and isthmus parts of oviduct on follicular and luteal phases. Songklanakarin Journal of Science and Technology, 42(4), 941-947.
Youngsabanant, M., Rabiab, S., Gumlungpat, N., and Panyarachun, B. (2019). In vitro characterization and viability of Vero cell lines supplemented with porcine follicular fluid proteins study. Science, Engineering and Health Studies, 13(3), 143-152.
Youngsabanant, M., and Rabiab, S. (2020). Potential Effect of porcine follicular fluid (pFF) from small-, medium-, and large-sized ovarian follicles on HeLa cell line viability. Science, Engineering and Health Studies, 14(2), 141-151.