Anti-dengue activity of synthetic peptides increases antiviral interferon-beta genes in LLC-MK2 cells

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Published: Aug 31, 2021
DOI: https://doi.org/10.14456/10.14456/sehs.2021.24
Keywords:
dengue peptides apoptosis caspase-10 interleukin-1beta tumor necrosis factor-alpha interferon-beta

Main Article Content

Jundee Rabablert
Department of Biology, Faculty of Science, Silpakorn University, Nakhon Pathom, Thailand. Corresponding author's e-mail: [email protected]
Promsin Masrinoul
Center for Vaccine Development, Institute of Molecular Biosciences, Mahidol University, Nakhon Pathom, Thailand
Sutee Yoksan
Center for Vaccine Development, Institute of Molecular Biosciences, Mahidol University, Nakhon Pathom , Thailand / Translational Research Unit, Chulabhorn Research Institute, Bangkok, Thailand
Supoth Rajakam
Center for Vaccine Development, Institute of Molecular Biosciences, Mahidol University, Nakhon Pathom, Thailand
Supanyika Sengsai
Department of Biology, Faculty of Science, Silpakorn University, Nakhon Pathom, Thailand
Korakot Changirakul
Department of Biology, Faculty of Science, Silpakorn University, Nakhon Pathom, Thailand
Prayad Sangngam
Department of Statistics, Faculty of Science, Silpakorn University, Nakhon Pathom, Thailand
Sunee Techaarpornkul
Department of Biopharmacy, Faculty of Pharmacy, Silpakorn University, Nakhon Pathom, Thailand
Sittiruk Roytrakul
Proteomics Research Laboratory, National Center for Genetic Engineering and Biotechnology, Khlong Luang, Pathum Thani, Thailand

Abstract

Dengue virus (DV) causes dengue diseases in human via the Aedes mosquitoes. Fatal cases of dengue hemorrhagic fevers show a complication of acute kidney injury. Previous reports revealed that synthetic DV2(413-440), DV2(413-447), and DV2(419-447) peptides inhibit the dengue envelope proteins in Aedes albopictus C6/36 cells, but nothing is known about the effect of these peptides on mammalian cells in relation to adaptive and innate immunities. In this study, the anti-dengue activity of these synthetic peptides in Rhesus monkey kidney LLC-MK2 cells was investigated at the cellular and molecular levels. Moreover, the effects of these synthetic peptides on apoptotic caspase-10, pro-inflammatory interleukin-1beta, tumor necrosis factor-alpha, and antiviral interferon-beta genes in LLC-MK2 cells were also sought. The results revealed the maximum non-toxic doses of DV2 (413-440), DV2 (413-447), and DV2 (419-447) in LLC-MK2 cells, with values of 77.88± 0.52, 47.01±1.32, and 63.82±1.97 µM, respectively. At 25 µM concentration, synthetic DV2 (413-447) and DV2 (419-447) peptides showed 100% plaque inhibition in simultaneous treatment. By contrast, these peptides showed ≤58% plaque inhibition in pre- and post-treatment at 7 days post-incubation. These synthetic peptides also inhibited Dengue 2 virus, apoptotic caspase-10, pro-inflammatory tumor necrosis factor-alpha, and interleukin-1beta genes. On the other hand, these peptides upregulated the antiviral interferon-beta gene in innate immunity. This study is the first report to reveal the anti-dengue activity of synthetic DV2 (413-440), DV2 (413-447), and DV2 (419-447) peptides in adaptive and innate immunity.

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How to Cite
Rabablert, J., Masrinoul, P., Yoksan, S., Rajakam, S., Sengsai, S., Changirakul, K., Sangngam, P., Techaarpornkul, S., & Roytrakul, S. (2021). Anti-dengue activity of synthetic peptides increases antiviral interferon-beta genes in LLC-MK2 cells. Science, Engineering and Health Studies, 15, 21030006. https://doi.org/10.14456/10.14456/sehs.2021.24
Issue
Volume 15, 2021
Section
Biological sciences

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