Evaluation of mannooligosaccharide production from konjac by using recombinant mannanase of Bacillus sp. SWU60 as a prebiotic in vitro and in vivo

Main Article Content

Kanoknat Woranuch
Nipaporn Sankasa
Tutsuji Sakamoto
Wasana Sukhumsirichart

Abstract

Prebiotics consist of non‐digestible foods that have many benefits to promote health. In the present study, mannooligosaccharide (MOS) produced from degradation of konjac by recombinant mannanase from Bacillus sp. SWU60 (rManS2) was investigated for its prebiotic properties both in vitro and in vivo. The prebiotic properties were evaluated by in vitro fermentation of the MOS (M2-M5) with Lactobacillus plantarum N25, and compared with the fermentation of the FOS. In the in vivo study, testing was performed on mice (BALB/cAJcl). The short-chain fatty acids (SCFAs) were evaluated both in vitro and in vivo. The results of the high -performance anion-exchange chromatography (HPAEC) indicated that the digested products were MOSs, including mannobiose, mannotriose, mannotetraose, and mannopentose. The antioxidant activity of MOS determined by a DPPH assay was 643.06 ± 0.05 µg/mL. These products promoted the growth of a probiotic strain, which was significantly different from the results of the control groups. In terms of the prebiotic effects in vivo, the results showed that all mice treated with MOS were normal, compared with the control group, and there were no deaths. The SCFAs, including acetate and lactate, were detected both in vitro and in vivo. In conclusion, the in vitro studies revealed that the MOS from konjac by rManS2 had a potential as an antioxidant, prebiotic property and had no toxic effect on mice.

Downloads

Download data is not yet available.

Article Details

Section
Biological sciences

References

Amna, K. S., Park, S. Y., Choi, M., Kim, S. Y., Yoo, A. Y., and Park, J. W. (2018). Antioxidant activity of manno-oligosaccharides derived from the hydrolysis of polymannan by extracellular carbohydrase of Bacillus N3. Journal of Marine Bioscience and Biotechnology, 10(1), 9-17.

Asano, I., Ikeda, Y., Fujii, S., and Iino, H. (2004). Effects of mannooligosaccharides from coffee on microbiota and short chain fatty acids in rat cecum. Food Science and Technology Research, 10(3), 273-277.

Baxter, N. T., Schmidt, A. W., Venkataraman, A., Kim, K. S., Waldron, C., and Schmidt, T. M. (2019). Dynamics of human gut microbiota and short-chain fatty acids in response to dietary interventions with three fermentable fibers. American Society for Microbiology, 10(1), 1-13.

Besten, G., Eunen, K., Groen, A. K., Venema, K., Reijngoud, D. J., and Bakker, B. M. (2013). The role of short-chain fatty acids in the interplay between diet, gut microbiota, and host energy metabolism. Journal of Lipid Research, 54(9), 2325-2340.

Bortolomeazzi, R., Verardo, G., Liessi, A., and Callea, A. (2010). Formation of dehydrodiisoeugenol and dehydrodieugenol from the reaction of isoeugenol with DPPH radical and their role in the radical scavenging activity. Food Chemistry, 118(2), 256-265.

Carlson, J. L., Erickson, J. M., Lloyd, B. B., and Slavin, J. L. (2018). Health effects and sources of prebiotic dietary fiber. Current Developments in Nutrition, 2(3), 1-8.

Castro-Alba, V., Lazarte, C., Perez-Rea, D., Carlsson, N., Almgren, A., Bergenstahl, B., and Granfeldt, Y. (2019). Fermentation of pseudocereals quinoa, canihua, and amaranth to improve mineral accessibility through degradation of phytate. Journal of the Science of Food and Agriculture, 99(11), 5239-5248.

Cao, P., Wu, L., Wu, Z., Pan, D., Zeng, X., Guo, Y., and Lian, L. (2019). Effects of oligosaccharides on the fermentation properties of Lactobacillus plantarum. Journal of Dairy Science, 102(4), 2863-2872.

Cheng, Z., Moore, J., and Yu, L. (2006). High-throughput relative DPPH radical scavenging capacity assay. Journal of Agricultural and Food Chemistry, 54(20), 7429-7436.

Dong, H., Rowland, I., and Yaqoob, P. (2012). Comparative effects of six probiotic strains on immune function in vitro. The British Journal of Nutrition, 108(3), 459-470.

Harvard Health. (2014). Helpful ways to strengthen your immune system and fight off disease. Harvard Health Publishing. [Online URL:https://www.health.harvard.edu/staying-healthy/how-to-boost-your-immune-system] accessed on April 6, 2020.

Jana, U. K., and Kango, M. (2020). Characteristics and bioactive properties of mannooligosaccharides derived from agro-waste mannans. International Journal of Biological Macromolecules, 149, 931-940.

Kahouli, I., Malhotra, M., Tomaro-Duchesneau, C., Saha, S., Marinescu, D., Rodes, L. S., Alaoui-Jamali, M. A., and Prakash, S. (2015). Screening and in-vitro analysis of Lactobacillus reuteri strains for short chain fatty acids production, stability and therapeutic potentials in colorectal cancer. Journal of Bioequivalence & Bioavailability, 7(1), 39-50.

Lafontaine, G. M. F., Fish, N. M., and Connerton, I. F. (2020). In vitro evaluation of the effects of commercial prebiotic GOS and FOS products on human colonic Caco-2 cells. Nutrients, 12(5), 1281.

Liu, Z., Ning, C., Yuan, M., Yang, S., Wei, X., Xiao, M., Fu, X., Zhu, C., and Mou, H. (2019). High-level expression of a thermophilic and acidophilic β-mannanase from Aspergillus kawachii IFO 4308 with significant potential in mannooligosaccharide preparation. Bioresource Technology, 295, 122257.

National Research Council. (2011). Guide for the care and use of laboratory animals eighth edition. National Institutes of Health (NIH), 1-246. [Online URL: https://grants.nih.gov/grants/olaw/guide-for-the-care-and-use-of-laboratory-animals.pdf] accessed on March 3, 2018.

Olorugbami, J. O., Gbadegesin, A. M., and Odunola, O. A. (2015). In vitro free radical scavenging and antioxidant properties of ethanol extract of Terminalia glaucescens. Pharmacognosy Research, 7(1), 49-56.

Pérez-Burillo, S., Pastoriza, S., Fernández-Arteaga, A., Luzon, G., Jiménez-Hernández, N., D'Auria, G., Francino, M. P., and Rufián-Henares, J. A. (2019). Spent coffee grounds extract, rich in mannooligosaccharides, promotes a healthier gut microbial community in a dose-dependent manner. Journal of Agricultural and Food Chemistry, 67(9), 2500-2509.

Peterson, C. T., Sharma, V., Uchitel, S., Denniston, K., Chopra, D., Mills, P. J., and Peterson, S. N. (2018). Prebiotic potential of herbal medicines used in digestive health and disease. The Journal of Alternative and Complementary Medicine, 24(7), 656-665.

Rizzoli, R. (2019). Nutritional influence on bone: role of gut microbiota. Aging Clinical and Experimental Research, 31(6), 743-751.

Rolim, P. (2015). Development of prebiotic food products and health benefits. Food Science and Technology (Campinas), 35(1), 3-10.

Rossi, M., Corradini, C., Amaretti, A., Nicolini, M., Pompei, A., Zanoni, S., and Matteuzzi, D. (2005). Fermentation of fructooligosaccharides and inulin by bifidobacteria: a comparative study of pure and fecal cultures. Applied and Environmental Microbiology, 71(10), 6150-6158.

Sabater, C., Prodanov, M., Olano, A., Corzo, N., and Montilla, A. (2016). Quantification of prebiotics in commercial infant formulas. Food Chemistry, 194, 6-11.

Seesom, W., Thongket, P., Yamamoto, T., Takenaka, S., Sakamoto, T., and Sukhumsirichart, W. (2017). Purification, characterization, and overexpression of an endo-1,4-β-mannanase from thermotolerant Bacillus sp. SWU60. World Journal of Microbiology and Biotechnology, 33(3), 53.

Singh, S., Ghosh, A., and Goyal, A. (2017). Manno-oligosaccharides as prebiotic-valued products from agro-waste. In Biosynthetic Technology and Environmental Challenges (Varjani, J. S., Binod, P., Kumar, S., and Khare, K. S., eds.), pp. 205-221. Singapore: Springer.

Suryawanshi, R. K., and Kango, N. (2021). Production of mannooligosaccharides from various mannans and evaluation of their prebiotic potential. Food Chemistry, 334, 1-9.

Thomas, D. W., and Greer, F. R. (2010). Clinical Report—Probiotics and Prebiotics in Pediatrics. American Academy of Pediatrics, 126(6), 1217-1231.

Thongsook, T., and Chaijamrus, S. (2018). Optimization of enzymatic hydrolysis of copra meal: compositions and properties of the hydrolysate. Journal of Food Science and Technology, 55(9), 3721-3730.

Thursby, E., and Juge, N. (2017). Introduction to the human gut microbiota. Biochemical Journal, 474(11), 1823-1836.

Torrecillas, S., Montero, D., and Izquierdo, M. (2014). Improved health and growth of fish fed mannan oligosaccharides: Potential mode of action. Fish & Shellfish Immunology, 36(2), 525-544.

Warburton, D. E. R., and Bredin, S. S. D. (2017). Health benefits of physical activity: a systematic review of current systematic reviews. Current Opinion in Cardiology, 32(5), 541-556.

Wolfe, A. J. (2015). Glycolysis for Microbiome Generation. Microbiology Spectrum, 3(3), 1-10.

Yamabhai, M., Sak-Ubol, S., Srila, W., and Haltrich, D. (2016). Mannan biotechnology: from biofuels to health. Critical Reviews in Biotechnology, 36(1), 32-42.

Yang, D., Yuan, Y., Wang, L., Wang, X., Mu, R., Pang, J., Xiao, J., and Zheng, Y. (2017). A review on konjac glucomannan gels: microstructure and application. International Journal of Molecular Sciences, 18(11), 2250.

Yazbeck, R., Lindsay, R. J., Geier, M. S., Butler, R. N., and Howarth, G. S. (2019). Prebiotics fructo-galacto-, and manno-oligosaccharide do not protect against 5-fluorouracil-induced intestinal mucositis in rats. The Journal of Nutrition, 149(12), 2164-2173.