Activity of methanolic Andrographis paniculate crude extract and its purified andrographolide against Japanese-encephalitis virus

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Published: Aug 31, 2021
DOI: https://doi.org/10.69598/sehs.15.21030007
Keywords:
Antiviral Flavivirus human neuroblastoma medicinal plant

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Jundee Rabablert
Department of Biology, Faculty of Science, Silpakorn University, Nakhon Pathom, Thailand. Corresponding author's e-mail: rabablert_j@su.ac.th
Juree Charoenteeraboon
Department of Biopharmacy, Faculty of Pharmacy, Silpakorn University, Nakhon Pathom, Thailand
Supathra Tiewchareon
Department of Parasitology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand
Natthapol Rabablert
Department of Biopharmacy, Faculty of Pharmacy, Silpakorn University, Nakhon Pathom, Thailand
Weesuda Makittithorn
Department of Biopharmacy, Faculty of Pharmacy, Silpakorn University, Nakhon Pathom, Thailand
Nantarat Chutipanyaporn
Department of Biology, Faculty of Science, Silpakorn University, Nakhon Pathom, Thailand
Supoth Rajchacham
Center for Vaccine Development, Institute of Molecular Science, Mahidol University, Nakhon Pathom, Thailand
Virach Junnu
Department of Parasitology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand
Sutee Yoksan
Center for Vaccine Development, Institute of Molecular Science, Mahidol University, Nakhon Pathom, Thailand

Abstract

Infection of Japanese encephalitis virus (JEV) generates brain swelling, namely Japanese encephalitis (JE) in human. This virus is spread by Culex spp. mosquitoes and causes 30% case-fatality rate in Asia and Western Pacific regions annually.  No specific cure for the disease is available. Andrographis paniculate methanolic extract and its purified andrographolide exhibit anti-inflammatory and antiviral properties. Our study is focused to antiviral activity of A. paniculate extracts and its elements against JEV in vitro. Half-maximal cytotoxicity (CC50) and the maximum non-toxic doses (MNTD) of A. paniculata in SK-NMC and LLC-MK2 are less than the CC50 and MNTD of andrographolide in SK-NMC and LLC-MK2 at 3 days-post treatment by MTT colorimetric assay. Both A. paniculata and andrographolide have antiviral effect by simultaneous treatment more than pre-treatment and post-treatment at 3 days-post treatment by time-of-addition assay. Antiviral action of these agents interfered virus attachment and had virucidal activity. The results indicated that A. paniculata and andrographolide have the potential antiviral activity against JEV.

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How to Cite
Rabablert, J., Charoenteeraboon, J., Tiewchareon, S., Rabablert, N. ., Makittithorn, W. ., Chutipanyaporn, N. ., Rajchacham, S. ., Junnu, V., & Yoksan , S. . (2021). Activity of methanolic Andrographis paniculate crude extract and its purified andrographolide against Japanese-encephalitis virus . Science, Engineering and Health Studies, 15, 21030007. https://doi.org/10.69598/sehs.15.21030007
Issue
Volume 15, 2021
Section
Biological sciences
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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

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