Novel approach to porcine epidemic diarrhea vaccine development by reverse vaccinology

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Published: Aug 10, 2022
DOI: https://doi.org/10.14456/sehs.2022.15
Keywords:
reverse vaccinology porcine epidemic diarrhea virus epitope vaccine mucosal antibody

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Woarawut Oniam
Department of Biomedicine and Health Informatics, Faculty of Pharmacy, Silpakorn University, Nakhon Pathom, Thailand
Suang Rungpragayphan
Department of Biomedicine and Health Informatics, Faculty of Pharmacy, Silpakorn University, Nakhon Pathom, Thailand
Busaba Powthongchin
Department of Biomedicine and Health Informatics, Faculty of Pharmacy, Silpakorn University, Nakhon Pathom, Thailand
Perayot Pamonsinlapatham
Department of Biomedicine and Health Informatics, Faculty of Pharmacy, Silpakorn University, Nakhon Pathom, Thailand. Corresponding author's e-mail: pamonsinlapa_p@su.ac.th

Abstract

Porcine epidemic diarrhea (PED) affects most swine, causing severe diarrhea and rapid death. The mortality rate of piglets reaches up to 80%-100%, which affects the pig industry. This study aimed to develop a PED vaccine using reverse vaccinology applying bioinformatic tools and evaluate epitope-based vaccines in an animal model. The genome of PED virus (PEDV)-CBR1 was retrieved and used, following the prediction of open reading frames (ORFs) by ORF finder. Consequently, protein localization was predicted by the iLoc-Virus program. Target proteins were subjected to adhesin-like prediction followed by B-cell epitope prediction. Finally, three epitope-based vaccines were synthesized and injected in an animal model, and the mucosal antibody response was measured. The candidate vaccine can stimulate mucosal antibodies in different mucosal organs. In conclusion, reverse vaccinology can be applied in PED cases by using different bioinformatic tools and proofing in the animal model.

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How to Cite
Oniam, W., Rungpragayphan, S., Powthongchin, B., & Pamonsinlapatham, P. (2022). Novel approach to porcine epidemic diarrhea vaccine development by reverse vaccinology. Science, Engineering and Health Studies, 16, 22050007. https://doi.org/10.14456/sehs.2022.15
Issue
Volume 16, 2022
Section
Health sciences
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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

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