Development of investigation method for Citrus reticulata Blanco cv. Sai-Nam-Phueng juice in bottle packaging by applying amino acid profiles
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Abstract
This research aimed to develop investigation method for real orange juice products in the market to appropriately obtain a tax exemption. The existing formol titration method is unable to fully identify nutrients or chemicals in the products, resulting in inaccurate tax exemption decisions. The present study applied the orange juice (Citrus reticulata Blanco cv. Sai-Nam-Phueng) amino acid profile as a reference profile, evaluated the orange juice products based on the bottle label information, and compared the results to the previous technique. To assess the amino acid profile of squeezed orange juice, high-performance liquid chromatography (HPLC) was used. Thirty orange juice samples were collected from market products around Bangkok and its vicinity in hermetically sealed packaging. The HPLC chroma-tograms demonstrated that six amino acids; glutamic acid, asparagine, serine, arginine, alanine, and valine, were substantially distinguishable at a confidence level of 95%. Aspartic acid was not found to be similarly distinguishable by HPLC. The amino acid profile could be used to inspect marketed orange juice products, distinguish real from fake orange juice, and determine the tax exemption.
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