Xylose-assimilating oleaginous yeasts from mangrove forest

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Patcharanan Amornrattanapan
Napatsawan Jhansiri

Abstract

The objectives of this research were to isolate and screen for oleaginous yeasts from leaves and soil samples at Mangrove Forest Conserve and Natural Study Center, Chonburi, Thailand. Investigation of biomass and lipid production of a selected isolated yeast was also conducted to explore the correlation of biomass and lipid production at different time courses. A total of 19 yeast isolates with xylose-assimilating ability were isolated and screened for their potential to accumulate lipid by Sudan black B staining and Nile red fluorescence assay. The results of Sudan black B staining showed that 9 yeast isolates had lipid accumulation in the cytoplasm of yeast cells, and isolate S3-2 was the most promising isolate for lipid accumulation based on the highest level of fluorescence intensity according to Nile red fluorescence assay. Thus, this yeast isolate was selected for the time-course study of biomass production, lipid production and lipid content. Biomass and lipid production achieved from isolate S3-2 were 175.56±1.13 mg/L and 42.59±0.33 mg/L, respectively. The maximum lipid content was determined to be 24.26±0.29% of dry cell weight. These results implied that oleaginous yeasts that could utilize xylose were distributed in mangrove ecosystem, and the strains isolated in this study could be used as a valuable lipid source for future relevant application.

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Biological sciences

References

Amornrattanapan, P., and Thongthep, P. (2019). Isolation and screening of oleaginous yeasts capable of using glycerol as a carbon source. Ramkhamhaeng Research Journal of Sciences and Technology, 22(2), 61-70.

Aristilde, L., Lewis, I. A., Park, J. O., and Rabinowitz, J. D. (2015). Hierarchy in pentose sugar metabolism in Clostridium acetobutylicum. Applied Environmental Microbiology, 81(4), 1452-1462.

Burdon, K. L. (1946). Fatty material in bacteria and fungi revealed by staining dried, fixed slide preparations. Journal of Bacteriology, 52(6), 665-678.

Castanha, R. F., Morais, L. A. S. D., Mariano, A. P., and Monteiro, R. T. R. (2013). Comparison of two lipid extraction methods produced by yeast in cheese whey. Brazilian Archives of Biology and Technology, 56(4), 629-636.

Chanklan, R., Kungkaew, P., Am-In, S., and Jindamorakot, S. (2012). Diversity of yeasts in the nature education center for mangrove conservation and ecotourism, Chonburi province. Thai Journal of Science and Technology, 1(3), 155-168. [in Thai]

Gao, Z., Ma, Y., Wang, Q., Zhang, M., Wang, J., and Liu, Y. (2016). Effect of crude glycerol impurities on lipid preparation by Rhodosporidium toruloides yeast 32489. Bioresource Technology, 218, 373-379.

Gusbeth, C. A., Eing, C., Gottel, M., Strabner, R., and Frey, W. (2016). Fluorescence diagnostics for lipid status monitoring of microalgae during cultivation. International Journal of Renewable Energy and Biofuels, 2016, 899698.

Jape, A., Harsulkar, A., and Sapre, V. R. (2014). Modified Sudan Black B staining method for rapid screening of oleaginous marine yeasts. International Journal of Current Microbiology and Applied Sciences, 3(9), 41-46.

Juanssilfero, A. B., Kaher, P., Amza, R. L., Miyamoto, N., Otsuka, H., Matsumoto, H., Kihira, C., Thontowi, A. Y., Ogimo, C., Prasetya, B., and Kondo, A. (2018). Effect of inoculum size on single-cell oil production from glucose and xylose using oleaginous yeast Lipomyces starkeyi. Journal of Bioscience and Bioengineering, 125(6), 695-702.

Lamers, D., Biezen, N. V., Martens, D., Peters, L., de Zilver, E. V., Dreumel, N. J., Wijffels, R. H., and Lokman, C. (2016). Selection of oleaginous yeasts for fatty acid production. BMC Biotechnology, 16(1), 45.

Munch, G., Sestric, R., Sparling, R., Levin, B. D., and Cicek, N. (2015). Lipid production in the under-characterized oleaginous yeasts, Rhodosporidium babjevae and Rhodosporidium diobovatum, from biodiesel-derived waste glycerol. Bioresource Technology, 185, 49-55.

Papanikolaou, S., and Aggelis, G., (2011). Lipid of oleaginous yeast. Part II: Technology and potential applications. European Journal of Lipid Science and Technology, 113(8), 1052-1073.

Patel, A., Antonopoulou, I., Enman, J., Rova, U., Christakopoulos, P., and Matsakas, L. (2019). Lipids detection and quantification in oleaginous microorganisms: an overview of the current state of the art. BMC Chemical Engineering, 1, 13.

Poontawee, R., Yongmanitchai, W., and Limtong, S. (2017). Efficient oleaginous yeasts for lipid production from lignocellulosic sugars and effects of lignocellulose degradation compounds on growth and lipid production. Process Biochemistry, 53(12), 44-60.

Siranonthana, N., Pratoomyot, J., Wattnachot, J., and Taweedet, S. (2015). Potential of marine microbes: As the source of essential fatty acids. Institute of Marine Science, Burapha University. [in Thai]

Sitepu, I. R., lgnatia, L., Franz, A. K., Wong, D. M., Faulina, S. A., Tsui, M., Kanti, A., and Boundy-Mills, K. (2012). An improved high-throughput Nile red fluorescence assay for estimating intracellular lipids in a variety of yeast species. Journal of Microbiological Methods, 91(2), 321-328.

Sitepu, I. R., Selby, T., Lin, T., Zhu, S., and Boundy-Mills, K. (2014). Carbon source utilization and inhibitor tolerance of 45 oleaginous yeast species. Journal of Industrial Microbiology and Biotechnology, 41(7), 1061-1070.

Surarit, W., Thaicharoen, P., Jindamorakot, S., and Chanklan, R. (2011). Screening of xylose-utilizing leaginous yeasts for lipid production. In The 23rd Annual Meeting of the Thai Society for Biotechnology “Systems Biotechnology: Quality & Success”, pp. 2-24. Bangkok, Thailand.

Tapia, E. V., Anschau, A., Coradini. A. L., Franco, T. T., and Deckmann, A. C. (2012). Optimization of lipid production by the oleaginous yeast Lipomyces starkeyi by random mutagenesis coupled to cerulenin screening. AMB Express, 2(1), 64.

Yamada, R., Yamauchi, A., Kashihara, T., and Ogino, H. (2017). Evaluation of lipid production from xylose and glucose/xylose mixed sugar in various oleaginous yeasts and improvement of lipid production by UV mutagenesis. Biochemical Engineering Journal, 128, 76-82.

Yu, X., Zheng, Y., Xiong, X., and Chen, S. (2014). Co-utilization of glucose, xylose and cellobiose by the oleaginous yeast Cryptococcus curvatus. Biomass and Bioenergy, 71, 340-349.