Experimental design for solid-liquid extraction from peanut kernel: Optimization through variability in antioxidant potential

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Kritamorn Jitrangsri
Amornrut Chaidedgumjorn
Malai Satiraphan


Extraction is a critical step in determining the actual content and related bioactivity potential of medicinal plants. This study aimed to develop optimized conditions for solid-liquid extraction (SLE) for the efficient isolating of phenolic substances and to accurately determine the antioxidant potential of peanut kernel. Relevant variables were studied through response surface methodology. During the initial screening step, 2-level full factorial designs were conducted. Independent variables included the percentage of ethanol content, solvent volume, temperature, and time for extraction significantly influenced SLE from the peanut kernels. Subsequently, the four significant factors were optimized by 2-level center-faced central composite design. Response surface plots showed significant correlation between independent variables and response variables. An increase in independent factor levels increased total phenolic content and antioxidant activity in a quadratic manner. The optimal conditions comprising 30% v/v ethanol content, 68 mL of extraction solvent, extraction temperature of 78°C, and extraction time of 153 min, were located at maximum antioxidant activity. The validity of the generated models was confirmed by the highest predicted R-square.


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Jitrangsri, K., Chaidedgumjorn, A., & Satiraphan, M. (2022). Experimental design for solid-liquid extraction from peanut kernel: Optimization through variability in antioxidant potential. Science, Engineering and Health Studies, 16, 22050015. https://doi.org/10.14456/sehs.2022.39
Health sciences


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