Isolation of Actinomycetes and xylanase production by using sugarcane bagasse as a substrate

Abstract

The purpose of this study was to select and identify of high xylanase activity producing Actinomycetes and to investigate its potential to produce enzyme xylanase utilizing sugarcane bagasse as a substrate. The Actinomycetes strains were selected from soil-sediment around wastewater pond from sugar production mills and soil in sugarcane fields, Ratchaburi. All selected strains were cultivated in basal medium supplementing sugarcane bagasse powder for 20 g/L and the crude enzyme was determined for xylanase activity. The results, three dominant-isolates were MCRU1 MCRU4 and MCRU19 exhibited high xylanase activity with 88.6±3.82 118.4±3.25 and 97.8±2.24 U/mL, respectively. Then, belonging to Streptomyces sp. family including Streptomyces sp. strain MCRU4 Streptomyces scabiei strain MCRU1 and Streptomyces sp. strain MCRU19, respectively. The MCRU4 strain was selected for study the potential to produce enzyme by using sugarcane bagasse powder as a substrate. The result showed that the xylanase activity and cell-protein were dramatically increased and the highest activity was at 96 h with 118.4±3.25 U/mL and was at 48 h with 0.25±0.12 g/L, respectively.