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Nucleotide sequences of nrITS were analyzed in order to evaluate genetic variation in 14 Thai waterlilies, Nymphaea spp. using BioEdit program. The ITS (internal transcribed spacer) region of approximately 900 bp contains sequences of partial 18S gene, ITS1, 5.8S gene, ITS2 and partial 26S gene. Nucleotide sequence variations at ITS1 and ITS2 were observed more than those at 18S, 5.8S and 26S genes. Comparison to that of ITS2, nucleotide length of ITS1 was longer, but GC contents were less. The 5.8S gene sequence was 156 bp in all 14 waterlily samples. Maximum likelihood (ML) phylogenetic tree of 14 waterlilies was constructed based on nrITS sequences using MEGA6 program with 1,000 replications (bootstrap). The tree could classify 14 waterlilies into 5 groups of different subgenus, i.e. subgenus Anecphya, subgenus Brachyceras, Intersubgeneric hybrids (ISG) of subgenus Nymphaea and subgenus Brachyceras, subgenus Lotos and subgenus Nymphaea. The classification was consistent with botanical classification in waterlily.
Keywords: waterlily; nrITS; phylogenetic tree
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