Phytochemicals and antioxidant capacities of Stephania sp. crude extract from Khanongpra subdistrict, Pak Chong District, Nakhon Ratchasima Province

Main Article Content

วนิดา ชูหมื่นไวย
ฐานิฏฐ์กานต์ ทวนไธสง
เพ็ญพร มีเงินลาด
ปิยสุดา เทพนอก
ปิยะธิดา กุศลรัตน์
วินัฐ จิตรเกาะ
รจนา เชื้อโคกกรวด
ขจรเดช เวียงสงค์
ชัยยุทธ ปิยวรนนท์

Abstract

The objectives of this research were to study antioxidant capacities by DPPH method and total phenolic compounds by Folin-Ciocalteu’s reagent. After interviewing some local wisdom from Khanongpra sub-district, Pak Chong District, Nakhon Ratchasima Province, we found that Stephania sp. bulbs have been used as an elixir plant and cosmetic proposes with no report. Stephania sp. bulbs were extracted with water and ethanol to give aqueous and ethanolic crude extract, respectively. The phytochemical test revealed flavonoids, saponin, tannin, and alkaloids in aqueous crude extract, and only alkaloids were found in ethanol extract. The different component results might come from the solubility of each crude extract. It was found that the amount of total phenolic compounds in Stephania sp. ethanol crude extract was 1,067.82 mg equivalent of gallic acid per 100 g of crude extract, which was higher than total phenolic compounds in the aqueous crude extract. Antioxidant activity test of these Stephania sp. crude extracts using DPPH method found that the IC50 of ethanol crude extract of Stephania sp. was lower than aqueous crude extract. These results were corresponding with the total phenolic compounds results by Folin-Ciocalteu’s reagent method. However, the IC50 of Stephania sp. ethanol crude extract was higher than BHA (Butylated hydroxyanisole), a positive control. According to its antioxidant activities, Stephania sp. could be a potential plant for the community to develop as a community product in the future.

Downloads

Download data is not yet available.

Article Details

How to Cite
ชูหมื่นไวย ว. ., ทวนไธสง ฐ. . ., มีเงินลาด เ. . ., เทพนอก ป., กุศลรัตน์ ป., จิตรเกาะ ว. ., เชื้อโคกกรวด ร., เวียงสงค์ ข., & ปิยวรนนท์ ช. . . (2021). Phytochemicals and antioxidant capacities of Stephania sp. crude extract from Khanongpra subdistrict, Pak Chong District, Nakhon Ratchasima Province. Thai Journal of Science and Technology, 10(1). https://doi.org/10.14456/tjst.2021.7
Section
วิทยาศาสตร์ชีวภาพ

References

Anggraini, T., Wilma, S., Syukri, D. and Azima, F., 2019, Total Phenolic, Anthocyanin, Catechins, DPPH Radical Scavenging Activity, and Toxicity of Lepisanthes alata (Blume) Leenh, Int J. Food Sci. 2019: 1-7.
Blanchfield, J. T., Sands, D. P., Kennard, C. H., Byriel, K. A. and Kitching, W., 2003, Characterisation of alkaloids from some Australian Stephania (Menispermaceae) species, Phytochemistry. 63(6): 711-720.
Buachoon, N. and Manjit, W., 2018, Quantitation of total phenolic antioxidant activity and development of skin care lotion, VRU Research and Development J. 13(2): 86-97. (in Thai)
Chatrthirananth, S., Sabayji, W. and Niyomthai, S., 2013, Phytochemical Screening and
Antioxidant Activity of Clerodendrum disparifolium Leaves, KKU Sci. J. 41(3): 723-730.
(in Thai)
Inthranuphakorn, R., 2004, Inspection and extraction of herbal essence, Chulalongkorn University, Bangkok, 215 p. (in Thai)
Maneechai, S. and Rinthong, P., 2016, Total phenolic and total flavonoid contents, free
radicalscavenging activity and tyrosinase inhibitory potential from the methanolic
extracts of Cajanus cajan (L.) Millsp. and Acacia concinna (Willd.) DC. Flowers.
KKU Sci. J. 44(1): 142-152. (in Thai)
Mojarrab, M., Nasseri, S., Hosseinzadeh, L. and Farahani, F., 2016, Evaluation of antioxidant
and cytoprotective activities of Artemisia ciniformis extracts on PC12 cell, Iran J. Basic
Med Sci. 19(4): 430-438.
Rattanamanee, K., 2014, Antiplatelet and antioxidative effects of Stephania venosa (Blume)
Spreng. Extract, Thai J. Pharmacol, 36(1): 36-34. (in Thai)
Semwal, D.K., Badoni, R., Semwal, R., Kothiyal, S. K., Singh, G. J. and Rawat U., 2010, The genus Stephania (Menispermaceae): Chemical and pharmacological perspectives, J. Ethnopharmacol. 132(2): 369-383.
Sharma, R., Chandan, G., Chahal, A. and Saini, R.V., 2017, Antioxidant and anticancer acitivity of methanolic extract from Stephania elegans, Int J. Pharm Pharm Sci. 9(2): 245-249.
Sutthisaksopon, P. and Rattamanee, C., 2019, Species diversity of undergrowth of deciduous
dipterocarp forest in Ban Phrao Community Forest Watthana Nakhon District, Sa Kaeo
Province, SWU. J. Sci. Technol. 11(22): 1-13. (in Thai)
Wacharakhupt, O., 2006, Radical Scavenging Agents, P.S. Print, Bangkok, 262 p. (in Thai)
Wuthi-udomlert, M. and Luanratana, O., 2003, Study of Thai medicinal herbs against dermatomycotic and opportunistic fungi, Department of Pharmacognosy, Bangkok, 196 p. (in Thai)