Encapsulation Properties and Digestibility of Encapsulated Aloe vera Bioactive Polysaccharides

Main Article Content

Phikunthong Auppasuk
Wipaporn Polklang
Rachata Lueachan
Thongkorn Ploypetchara
Siriporn Budseekoad
Sinee Siricoon
Wiriyaporn Sumsakul
Chiramet Auranwiwat
Waraporn Sorndech

Abstract

Aloe vera is the famous medicinal plant that has been used in the treatment of many diseases. New findings showed that Aloe vera extract can stimulate the osteoblast differentiation in human fetal osteoblast cells which can potentially be used as functional ingredient for increasing bone mass. This study aimed to contribute to this growing area of functional ingredient related to non-communicable diseases (NCDs) research by exploring the effect of encapsulation wall-materials on encapsulation properties and digestibility of Aloe vera extracts. Aloe vera extract (2.0% wv-1) were encapsulated with 7.5% (wv-1) maltodextrin (MD), 7.5% (wv-1) gum arabic (GA) and their mixtures (5% (wv-1) MD + 2.5% (wv-1) GA and 2.5% (wv-1) MD + 5% (wv-1) GA) at 50 oC for 16 h using hot air dryer. The results of the encapsulation efficiency (EE) showed that an increase in GA content could increase encapsulation efficiency as compared to MD-only wall-material. GA-only wall-material provided the highest EE (94.96%) while the MD only provided the lowest EE (68.71%). The appearance of encapsulated extract displayed an increase in yellow color when increasing GA content which was corresponded to an increase in positive b* value. The encapsulated microstructure of all samples showed smooth surface, angular and fragmentation structures indicated a break down in the encapsulated structure after drying.  There was a significant positive correlation between an increase in GA content and antioxidant activity. Moreover, an increase in GA content as wall-materials showed restricted reducing sugar release following hydrolysis by porcine pancreatic α-amylase, pepsin, pancreatin and glucoamylase. Hence, GA could extend a release of bioactive compounds during digestion. These data led to an enhanced understanding of encapsulated bioactive compounds delivery and how encapsulation wall materials and Aloe vera extract affected the encapsulated physicochemical properties and digestibility. Further research should be undertaken to explore how bioactive compounds and wall-materials interaction target delivery and control bioactive compounds release which will be a pivotal role in the future of personalized diet.

Article Details

How to Cite
Auppasuk, P. ., Polklang, W., Lueachan, R. ., Ploypetchara, T. ., Budseekoad, S. ., Siricoon, S., Sumsakul, W. ., Auranwiwat, C. ., & Sorndech, W. (2024). Encapsulation Properties and Digestibility of Encapsulated Aloe vera Bioactive Polysaccharides . Thai Journal of Science and Technology, 10(6), 677–690. https://doi.org/10.14456/tjst.2021.51
Section
วิทยาศาสตร์ชีวภาพ

References

Baruah, A., Bordoloi, M., & Deka Baruah, H. P. (2016). Aloe vera: A multipurpose industrial crop. Industrial Crops and Products, 94, 951-963.

Hęś, M., Dziedzic, K., Górecka, D., Jędrusek-Golińska, A., & Gujska, E. (2019). Aloe vera (L.) webb.: Natural sources of antioxidants - A review. Plant Foods for Human Nutrition, 74(3), 255-265.

Khorasani, G., Hosseinimehr, S. J., Azadbakht, M., Zamani, A., & Mahdavi, M. R. (2009). Aloe versus silver sulfadiazine creams for second-degree burns: A randomized controlled study. Surgery Today, 39, 587–591.

Chantarawaratit, P., Sangvanich, P., Banlunara, W., Soontornvipart, K., & Thunyakitpisal, P. (2013). Acemannan sponges stimulate alveolar bone, cementum and periodontal ligament regeneration in a canine class II furcation defect model. Journal of Periodontal Research, 49, 164–178.

Soonsit, O., Suksiriphattanapong, B., Sorndech, W., & Kasekarn, W. (2021). Effects of Noni and Morinda citrifolia (Noni) extracts on cell proliferation and alkaline phosphatase activity in human fetal osteoblast cell line. In The 7th International Conference on Biochemistry and Molecular Biology (BMB 2021) "Challenges in Biochemistry to Overcome Pandemics and Disruptions" (pp. 116-123). Bangkok, Thailand: Kasetsart University. (in Thai)

Ratanasiriwat, P., Worawattanamateekul, W., & Klaypradit, W. (2013). Properties of encapsulated wasabi flavour and its application in canned food. International Journal of Food Science, 48(4), 749-757.

Somogyi, M., & Nelson, N. (1944). A photometric adaptation of the Somogyi method for the determination of glucose. Journal of Biological Chemistry, 153(2), 375–380.

Prompiputtanapon, K., Sorndech, W., & Tongta, S. (2020). Surface modification of tapioca starch by using the chemical and enzymatic method. Starch - Stärke, 72(3-4), 1900133.

Li, J., & Corke, H. (1999). Physicochemical properties of normal and waxy Job’s tears (Coix lachryma-jobi L.) starch. Cereal Chemistry, 76, 413-416.

Sopade, P. A., & Gidley, M. J. (2009). A rapid in-vitro digestibility assay based on glucometry for investigating kinetics of starch digestion. Starch - Stärke, 61(5), 245-255.

Na-Nakorn, K., Hamaker, B. R., & Tongta, S. (2021). Physicochemical and rheological properties of cooked extruded reformed rice with added protein or fiber. LWT, 151, 112196.

Suyalek, S., Jaturonglumlert, S., Amornlerdpison, D., Narkprasom, N., & Narkprasom, K. (2019). Encapsulation of crude extracts from banana (Musa X paradisca) flowers by spray drying. Burapha University Science Journal, 25(2), 448-463. (in Thai)

Alves, S. F., Borges, L. L., dos Santos, T. O., de Paula, J. R., Conceição, E. C., & Bara, M. T. F. (2014). Microencapsulation of essential oil from fruits of Pterodon emarginatus using gum arabic and maltodextrin as wall materials: Composition and stability. Drying Technology, 32(1), 96-105.

Ratanasiriwat, P., Pai-Ngam, K., Sa-Ngonwong, R., Chaonatri, W., & Pienchob, P. (2015). Encapsulation of crude extracts from pomelo peel. RMUTTO Research Journal, 1-12. (in Thai)

Rithmanee, T., & Phonpannawit, A. (2018). Anthocyanin encapsulation from purple corncob by spray drying. EAU Heritage Journal, 12(2), 169-180. (in Thai)

Khunthawad, A., & Sripui, J. (2013). Effects of encapsulation by spray drying on physical properties of Mamao (Antidesma thwaitesianum) powder. In Graduate Research Conference Khon Kaen University, PMP14 (pp. 386-391). (in Thai)

Mirghani, M., Elnour, A., Kabbashi, N., Alam, M., Musa, K. H., & Abdullah, A. (2018). Determination of antioxidant activity of gum Arabic: An exudation from two different locations. Scientific, 44, 179.

Mohd Zin, Z., Razman, N. H., Mamat, H., Manap, M. N., & Zainol, K. (2021). The influence of Gum Arabic on the physicochemical and antimicrobial activity of the microencapsulated Mahkota Dewa (Phaleria macrocarpa) leaves. Food Research, 5, 203-213.

Mahendran, T., Williams, P. A., Phillips, G. O., Al-Assaf, S., & Baldwin, T. C. (2008). New insights into the structural characteristics of the arabinogalactan−protein (AGP) fraction of gum arabic. Journal of Agricultural and Food Chemistry, 56(19), 9269-9276.

Sensoy, I. (2021). A review on the food digestion in the digestive tract and the used in vitro models. Current Research in Nutrition and Food Science, 4, 308-319.

Peanparkdee, M., Borompichaichartkul, C., & Iwamoto, S. (2021). Bioaccessibility and antioxidant activity of phenolic acids, flavonoids, and anthocyanins of encapsulated Thai rice bran extracts during in vitro gastrointestinal digestion. Food Chemistry, 361, 130161.