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This research aimed to evaluate the effect of carbon and nitrogen sources on the production of cell mass and omega-3 fatty acids by thraustochytrids and phylogenetic relationships of the potential isolates based on their nucleotide sequences. Thraustochytrids were studied for biomass and omega-3 fatty acids production by using 3 and 6 % glucose concentrations supplemented with nitrogen from different sources, including 1 % yeast extract, 1 % yeast extract and peptone, and 1 % yeast extract, peptone and tryptone. To assess the effects of these different treatments, we determined the cell mass and omega-3 fatty acids concentration by dried cells weight and gas chromatography–mass spectrometry, respectively. The maximum dried cell weight (32.87 g/L) was obtained from isolate TC9-TSU using initial concentrations of 6 % glucose and 1 % yeast extract, peptone and tryptone. Under these conditions, this isolate gave the highest level of PUFAs production (52.56 %), with DHA (docosahexaenoic acid, 22:6n3), DPA (docosapentaenoic acid, 22:5n3), and EPA (eicosapentaenoic acid, 20:5n3). In addition, the potential isolate TC9-TSU was identified based on 18S rDNA nucleotide sequences as Aurantiochytrium limacinum (accession number MG279107). This result showed that thraustochytrids are an interested alternative source of omega-3 polyunsaturated fatty acids production for further application.
Keywords: thraustochytrids; cell mass; omega-3 fatty acid
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