สภาวะสารอาหารที่เหมาะสมสำหรับการผลิตมวลเซลล์และโอเมก้า-3 จากทรอสโทไคทริดส์

Main Article Content

พฤทฐิภร ศุภพล
ชุติมา แก้วพิบูลย์
สิตา ปรีดานนท์

Abstract

This research aimed to evaluate the effect of carbon and nitrogen sources on the production of cell mass and omega-3 fatty acids by thraustochytrids and phylogenetic relationships of the potential isolates based on their nucleotide sequences. Thraustochytrids were studied for biomass and omega-3 fatty acids production by using 3 and 6 % glucose concentrations supplemented with nitrogen from different sources, including 1 % yeast extract, 1 % yeast extract and peptone, and 1 % yeast extract, peptone and tryptone. To assess the effects of these different treatments, we determined the cell mass and omega-3 fatty acids concentration by dried cells weight and gas chromatography–mass spectrometry, respectively. The maximum dried cell weight (32.87 g/L) was obtained from isolate TC9-TSU using initial concentrations of 6 % glucose and 1 % yeast extract, peptone and tryptone. Under these conditions, this isolate gave the highest level of PUFAs production (52.56 %), with DHA (docosahexaenoic acid, 22:6n3), DPA (docosapentaenoic acid, 22:5n3), and EPA (eicosapentaenoic acid, 20:5n3). In addition, the potential isolate TC9-TSU was identified based on 18S rDNA nucleotide sequences as Aurantiochytrium limacinum (accession number MG279107). This result showed that thraustochytrids are an interested alternative source of omega-3 polyunsaturated fatty acids production for further application. 


Keywords: thraustochytrids; cell mass; omega-3 fatty acid

Article Details

Section
Biological Sciences
Author Biographies

พฤทฐิภร ศุภพล

สาขาวิชาชีววิทยา คณะวิทยาศาสตร์ มหาวิทยาลัยทักษิณ วิทยาเขตพัทลุง ตำบลบ้านพร้าว อำเภอป่าพะยอม จังหวัดพัทลุง 93110

ชุติมา แก้วพิบูลย์

สาขาวิชาชีววิทยา คณะวิทยาศาสตร์ มหาวิทยาลัยทักษิณ วิทยาเขตพัทลุง ตำบลบ้านพร้าว อำเภอป่าพะยอม จังหวัดพัทลุง 93110

สิตา ปรีดานนท์

ศูนย์พันธุวิศวกรรมและเทคโนโลยีชีวภาพแห่งชาติ (ไบโอเทค) ตำบลคลองหนึ่ง อำเภอคลองหลวง จังหวัดปทุมธานี 12120

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