Effects of Phe and JA on Growth and Secondary Metabolite Contents of Roselle Callus (Hibiscus sabdariffa L.) under Aseptic Conditions
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Abstract
Precursors and elicitors are able to enhance secondary metabolite contents in several medicinal plant species. The objective of this study was to investigate the effect of phenylalanine (Phe, a precursor) and jasmonic acid (JA, an elicitor) on secondary metabolite contents of Roselle callus (Hibiscus sabdariffa L. accession HS005). The dark-red-colored-callus was cultured on MS medium supplemented with 4.52 µM 2,4-D and 4.44 µM BA in combination with 50-200 µM Phe or 50-150 µM JA for 14 days. The result showed that JA was more effective in stimulating the accumulation of flavonoid compounds than Phe. The highest contents of total anthocyanin (0.47±0.11 mg cyd-3-glu/L), total phenolic compounds (55.14±.6.59 mg GAE/g dry extract) and flavonoids (30.74±6.31 mg CE/g dry extract) were obtained in 100 µM JA callus which were 5.22, 10.36 and 7.82 folds higher than the control treatment, respectively. The greatest DPPH radical scavenging activity with EC50 of 39.28±4.79 µg/mL also occurred in 100 µM JA.
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