Effect of Yeast Extract Elicitation Period on Secondary Metabolite Contents of in vitro Dioscorea birmanica Prain & Burkill Shoots

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เยาวพา จิระเกียรติกุล
รัชนีวรรณ จิระพงศ์พัฒนา
ภาณุมาศ ฤทธิไชย
ศรีโสภา เรืองหนู
อรุณพร อิฐรัตน์

Abstract

Yeast extract (YE) is an elicitor which can enhance secondary metabolite contents in many medicinal plants. Therefore, the objective of this study was to investigate effect of YE elicitation period on total saponin content, total phenolic content and DPPH radical scavenging activity of Dioscorea birmanica Prain & Burkill shoot under aseptic conditions. Node explants were cultured on MS medium supplemented with or without 3 g/L YE for 1, 2, 4 and 6 weeks. The experiment was arranged in 2 x 4 factorial in Completely Randomized Design (CRD). The results revealed that there was an interaction between YE supplementation and elicitation period on total saponin content. The highest total saponin content of 1,233.33±47.15 µg diosgenin/g dry extract or 1.93 times of non YE shoots was achieved when the shoots were grown on MS medium supplemented with 3 g/L YE for 2 weeks. However, YE had no effect on total phenolic content and DPPH radical scavenging activity.

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วิทยาศาสตร์ชีวภาพ
Author Biographies

เยาวพา จิระเกียรติกุล

สาขาวิชาเทคโนโลยีการเกษตร คณะวิทยาศาสตร์และเทคโนโลยี มหาวิทยาลัยธรรมศาสตร์ ศูนย์รังสิต ตำบลคลองหนึ่ง อำเภอคลองหลวง จังหวัดปทุมธานี 12120

รัชนีวรรณ จิระพงศ์พัฒนา

สาขาวิชาเทคโนโลยีการเกษตร คณะวิทยาศาสตร์และเทคโนโลยี มหาวิทยาลัยธรรมศาสตร์ ศูนย์รังสิต ตำบลคลองหนึ่ง อำเภอคลองหลวง จังหวัดปทุมธานี 12120

ภาณุมาศ ฤทธิไชย

สาขาวิชาเทคโนโลยีการเกษตร คณะวิทยาศาสตร์และเทคโนโลยี มหาวิทยาลัยธรรมศาสตร์ ศูนย์รังสิต ตำบลคลองหนึ่ง อำเภอคลองหลวง จังหวัดปทุมธานี 12120

ศรีโสภา เรืองหนู

สถานการแพทย์แผนไทยประยุกต์ คณะแพทยศาสตร์ มหาวิทยาลัยธรรมศาสตร์ ศูนย์รังสิต ตำบลคลองหนึ่ง อำเภอคลองหลวง จังหวัดปทุมธานี 12120

อรุณพร อิฐรัตน์

สถานการแพทย์แผนไทยประยุกต์ คณะแพทยศาสตร์ มหาวิทยาลัยธรรมศาสตร์ ศูนย์รังสิต ตำบลคลองหนึ่ง อำเภอคลองหลวง จังหวัดปทุมธานี 12120

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