Main Article Content
Grape (Vitis vinifera) is a fruit with antioxidant properties because of polyphenols compounds, the important secondary metabolites. Polyphenols can inhibit the causes of various types of cancer and also reduce cholesterol, reducing the risk of heart disease. Therefore, this research aims to study the effect of plant growth regulators on callus induction and growth for secondary metabolite production by cell culture technique. The effect of growth regulators on the induction and growth of callus from grape explants which cultured on Murashige and Skoog (MS) supplemented with growth regulators as N6-benzyladenine (BAP) and napthaleneacetic acid (NAA). From the result, MS supplemented with BAP and NAA at concentrations of 1 and 0.5 mg/L, respectively can induce callus formation. Moreover, the effects of BAP at different concentrations on callus growth at 4 weeks were studied. The MS medium supplemented with BAP at 1.5 mg/L was suitable for grape callus growth which showed the highest of fresh weight, 3.70±0.53 g. Then the callus extract was analysis of compounds through TLC techniques. The result showed that grape callus comprised of resveratrol and exhibited DPPH radical scarvenging activity EC50 of 21.38 µg/ml.
Keywords: grape; callus; growth regulator; secondary metabolite; antioxidant activity
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