Evaluation of the Biological Activities of Sung Yod Germinated Brown Rice Extract Soaking in Pandanus Leaf Extract

Main Article Content

ชุติมา แก้วพิบูลย์
ณวงศ์ บุนนาค

Abstract

Nowadays, the consumption of germinated brown rice is becoming a popular health food because of their high nutritional value. Therefore, the aim of this research was to study antioxidant and biological activities of the Sung Yod germinated brown rice (SGBr) extract which was soaked in Pandanus leaf extract (PE), i.e. the antioxidant activities assay by DPPH and ABTS methods, inhibitory activity of tyrosinase, and cytotoxicity assay against HaCaT cell lines with MTT method. From the results, SGBr extract showed total phenolic content of 134.52 mg GAE/g extract. The antioxidant activity assays by DPPH and ABTS method showed EC50 values of 53.42 and 9.92 µg/mL, respectively, which were more effective than control significantly at the confidence level of 95 %. In addition, the SGBr extract showed the inhibitory activity of tyrosinase with IC50 value of 3.14 µg/mL. Moreover, cytotoxicity assay against HaCaT cell lines showed IC50 values of more than 400 µg/mL. It is very interesting for the development of the SGBr extract as a cosmetic product.


 

Article Details

Section
Biological Sciences
Author Biographies

ชุติมา แก้วพิบูลย์

สาขาวิชาชีววิทยา คณะวิทยาศาสตร์ มหาวิทยาลัยทักษิณ วิทยาเขตพัทลุง ตำบลบ้านพร้าว อำเภอป่าพะยอม จังหวัดพัทลุง 93210

ณวงศ์ บุนนาค

สาขาวิชาวิทยาศาสตร์และคณิตศาสตร์พื้นฐาน คณะวิทยาศาสตร์ มหาวิทยาลัยทักษิณ วิทยาเขตสงขลา ตำบลเขารูปช้าง อำเภอเมือง จังหวัดสงขลา 90000

References

Liu, L., Guo, J., Zhang, R., Wei, Z., Deng, Y., Guo, J. and Zhang, M., 2015, Effect of degree of milling on phenolic profiles and cellular antioxidant activity of whole brown rice, Food Chem. 185: 318-325.

Sompong, R., Siebenhandl-Ehn, S., Linsberger-Martin, G. and Berghofer, E., 2011, Physicochemical and antioxidative properties of red and black rice varieties from Thailand, China and Sri Lanka, Food Chem. 124: 132-140.

Sutharut, J. and Sudarat, J., 2012, Total anthocyanin content and antioxidant activity of germinated colored rice, Int. Food Res. J. 19: 215-221.

Thammapat, P., Meeso, N. and Siriamornpun, S., 2015, Effects of NaCl and soaking temperature on the phenolic compounds, alpha-tocopherol, gamma-oryzanol and fatty acids of glutinous rice, Food Chem. 175: 218-24.

Moongngarm, A. and Saetung, N., 2010, Comparison of chemical compositions and bioactive compounds of germinated rough rice and brown rice, Food Chem. 122: 782-788.

Chen, H.H., Chang, H.C., Chen, Y.K., Hung, C.L., Lin, S.Y. and Chen, Y.S., 2016, An improved process for high nutrition of germinated brown rice production: Low-pressure plasma, Food Chem. 191: 120-127.

Ghasemzadeh, A. and Jaafar, H.Z.E., 2013, Profiling of phenolic compounds and their antioxidant and anticancer activities in pandan (Pandanus amaryllifolius Roxb.) extracts from different locations of Malaysia, BMC Comp. Altern. Med. 13: 341-341.

Prior, R.L., Wu, X. and Schaich, K., 2005, Standardized Methods for the determination of antioxidant capacity and phenolics in foods and dietary supplements, J. Agric. Food Chem. 53: 4290-4302.

Floegel, A., Kim, D.O., Chung, S.J., Koo, S.I. and Chun, O.K., 2011, Comparison of ABTS/DPPH assays to measure antioxidant capacity in popular antioxidant-rich US foods, J. Food Comp. Anal. 24: 1043-1048.

Sangsila, A., Promden, W. and Pimda, W., 2018, Antioxidant and antityrosinase activities in germinated brown rice of indigenous Thai cultivars, Int. J. Agric. Tech. 14: 1883-1892.

Bulanawichit, W., Thanakon, K. and Tantip, B., 2018, Effects of brown rice and germinated brown rice extracts from Thai rice cultivars (PL2 and KDML105) on adipogenic, adipocytokine, and antioxidant genes in 3T3-L1 adipocytes, CMU J. Nat. Sci. 17: 79-96.