ฤทธิ์ยับยั้งเอนไซม์แอลฟาอะไมเลสและฤทธิ์ต้านออกซิเดชันของสารสกัดหยาบจากผลมะเดื่ออุทุมพรด้วยเอทานอล
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Abstract
Abstract
Diabetes mellitus (DM) is a metabolic disorder which can lead to chronic hyperglycemia. Diabetes impairs blood vessels and increases the risk of chronic kidney disease, vision loss and heart attack. The increase of free radical production is commonly related to these complications. Inhibition of carbohydrate hydrolyzing enzymes such as a-amylase, in the gastrointestinal tract can be an effective strategy to slow down dietary carbohydrate digestion and also reduce postprandial hyperglycemia. The objectives of this study were to investigate the a-amylase inhibition activity of Ficus racemosa Linn. fruit ethanolic extract (FRFE) and to determine its antioxidant capacity. The extract was determined for a-amylase inhibitory effect by 3,5 dinitrosalicylic (DNS) method, and for antioxidant potentials by radical scavenging capacity assay (2,2-diphenyl-1-picrylhydrazyl, DPPH) and 2,2’-azinobis (3-ethylbenzothiazoline-6-sulfonic acid, ABTS), and ferric ion reducing power assay (FRAP). In DNS method, the highest inhibitory effect of the extract against a-amylase was 84.02±3.17 % at 20 µg/mL with the IC50 value of 7.44±0.23 µg/mL. In DPPH and ABTS assays, FRFE had IC50 value of 128.84±52.89 µg/mL and 30.21±2.42 µg/mL, respectively. In FRAP assay, FRFE had FRAP value of 0.58±0.03 mM Fe2+/mg dried extract weight. In conclusion, FRFE demonstrated an a-amylase inhibitory activity and antioxidant potentials. However, this study suggests that it would be further investigated, especially active the therapeutically ingredients of this extract as well as clinical study to develop a novel anti-diabetic drug.
Keywords: fruit of Ficus racemose; alpha-amylase; antioxidant
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References
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